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M8662

Sigma-Aldrich

矿物油

PCR Reagent

别名:

Mineral oil for PCR

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About This Item

CAS号:
8042-47-5
EC號碼:
232-455-8
MDL號碼:
MFCD00131611
分類程式碼代碼:
12181504
NACRES:
NA.52
等級:
PCR Reagent
技術:
PCR: suitable

等級

PCR Reagent

品質等級

100

形狀

liquid

包裝

vial of 1.5 mL (Total volume 7.5 mL (5 vials))

技術

PCR: suitable

顏色

colorless

折射率

n20/D 1.467 (lit.)

密度

0.84 g/mL at 25 °C (lit.)

異物活動

DNase, RNase, protease, none detected

儲存溫度

room temp

InChI 密鑰

AEOVEGJBKQQFOP-DDVLFWKVSA-L

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相关类别

一般說明

矿物油可用作聚合酶链反应（PCR） 中的反应混合物覆盖层，以防止在没有加热盖的 PCR 仪器的热循环过程中反应溶液蒸发。

應用

矿物油已用于:
  • 常规PCR扩增
  • 逆转录聚合酶链反应 （RT–PCR） 扩增工艺，提高设备和循环仪之间的导热性
  • 防止在 Bravo 自动液体处理平台上进行的单细胞 MATQ 测序过程中蒸发

特點和優勢

  • 以5 × 1.5 mL（1 瓶）包装规格提供，便于使用
  • 经测试不含 RNase、DNase和蛋白酶。

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

No data available

閃點（°C）

No data available

個人防護裝備

Eyeshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
MKCN0398
MKCK1218
MKCF5776
MKCC0168
MKBQ6288V

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密度标准品 870kg/m3 H&D Fitzgerald Ltd. Quality

Supelco

76731

密度标准品 870kg/m3

vibrant-m

RTM3

矿物油旋转粘度标准

vibrant-m

RTM1

矿物油旋转粘度标准

石蜡油 puriss., meets analytical specification of Ph. Eur., BP, viscous liquid

Sigma-Aldrich

18512

石蜡油

Hartmut Kaiser et al.
Plant physiology, 143(2), 1068-1077 (2006-12-13)
The response of stomata to a reduction of air humidity is composed of a hydropassive opening followed by active closure. Whereas the mechanisms behind the hydropassive opening are largely understood, the location and physiological basis of the sensing mechanisms leading
Ivan K Dimov et al.
Nature communications, 5, 3451-3451 (2014-03-29)
Discriminating cellular heterogeneity is important for understanding cellular physiology. However, it is limited by the technical difficulties of single-cell measurements. Here we develop a two-stage system to determine cellular heterogeneity. In the first stage, we perform multiplex single-cell RNA cytometry
Hsin-I Jen et al.
eLife, 8 (2019-04-30)
The mammalian cochlea loses its ability to regenerate new hair cells prior to the onset of hearing. In contrast, the adult vestibular system can produce new hair cells in response to damage, or by reprogramming of supporting cells with the
Neus Bota-Rabassedas et al.
Cell reports, 35(3), 109009-109009 (2021-04-22)
Cancer cells function as primary architects of the tumor microenvironment. However, the molecular features of cancer cells that govern stromal cell phenotypes remain unclear. Here, we show that cancer-associated fibroblast (CAF) heterogeneity is driven by lung adenocarcinoma (LUAD) cells at
Keith E Herold et al.
Methods in molecular biology (Clifton, N.J.), 504, 441-458 (2009-01-23)
A prototype handheld, compact, rapid thermocycler was developed for multiplex analysis of nucleic acids in an inexpensive, portable configuration. Instead of the commonly used Peltier heating/cooling element, electric thin-film resistive heater and a miniature fan enable rapid heating and cooling
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实验方案

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Hot Start Taq Polymerase protocol to reduce non-specific amplification, with MgCl2 Optimization

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Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Amplification of DNA Using Jumpstart™ Taq DNA Polymerase (D9307)

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

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