Hs 27

94041901

• 分類程式碼代碼: 41106514

属性

• 生物源: human prostate
• 增長模式: Adherent
• 染色體組型: 46XY
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human foreskin, fibroblast

細胞系描述

Hs27 is one of a series of human foreskin fibroblast lines developed at NBL, California. The material was obtained from a normal new-born, and the cells possess the G6PD type A phenotype. Longevity studies carried out at NBL demonstrated that the cells can be propagated for 42 passages. There were no consistent aberrations in analysed cells. Ethnicity: Black.

培養基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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