HT29-MTX-E12

12040401, human colon, Epithelial

• 别名: HT-29-MTX-E12 Cells, HT29, HT29 Cells, HT29-MTX, HT29-MTX Cells, MTX-E12 Cells
• 分類程式碼代碼: 41106514

属性

• product name: HT29-MTX-E12, 12040401, from human colon
• 生物源: human colon
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

HT29, HT29-MTX, methotrexate, mucous layer, intestinal permeation, oral drug absorption

細胞系描述

HT29 cells were differentiated into mature goblet cells using methotrexate. Mucous-secreting HT29-MTX subclones were isolated from this cell clone and characterized with regard to tight junction formation, development of confluent monolayers and productio

DNA分析

STR-PCR Data:
Amelogenin: X
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6
TPOX: 8,9
vWA: 17

培養基

DMEM + 2mM Glutamine + 1% Non essential amino acids + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split subconfluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin/EDTA; 7.5-10% CO_2; 37 °C. Suggested seeding density 2-4x10,000 cells/cm^².

其他說明

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