T84 Cell Line human

88021101, human colon (lung metastasis), Epithelial

• 别名: T-84 Cells
• 分類程式碼代碼: 41106514

属性

• 产品名称: T84 Cell Line human, 88021101
• 生物源: human colon (lung metastasis)
• 描述: Human colon carcinoma
• 增長模式: Adherent
• 染色體組型: 2n = 46, modal no. 56
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: metastasis
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human colon carcinoma

細胞系描述

Derived from a lung metastasis of colon carcinoma in a 72 year old male. Tumour tissue was inoculated sub-cutaneously and serially transplanted in BALB/c nude mice and subsequently established in in vitro culture. The histological characteristics of the tumour were maintained throughout the transplantation procedure. They have receptors for a range of peptide hormones and neurotransmitters. The cells grow as monolayers, exhibit tight junctions and desmosomes between adjacent cells.

應用

Human T84 cell line has been used for comparative genome analysis of Salmonella enterica serovar Typhimurium definitive type 2 (DT2) isolate 94-213 and S. Typhimurium isolates SL1344, DT104 and D23580. The ability of these isolates to invade culture medium has also been studied. It has also been used to construct and study the suitability of an in vitro model to study chemotherapy-induced mucosal damage.

Tumourigenicity, neurotransmitter and hormone studies

培養基

Ham′s F12 + DMEM (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 - 1:4 i.e. seeding at 1-3 x 10,000 cells / cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Cells grow slowly and may not form a complete monolayer, maintain at high density with a minimum of 25% confluency.

其他說明

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