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Merck

M8403

Sigma-Aldrich

McCoy 5A培养基

Modified, with sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

别名:

5A basal medium

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About This Item

EC號碼:
MDL號碼:
分類程式碼代碼:
12352207
NACRES:
NA.75

品質等級

無菌

sterile-filtered

形狀

liquid

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

成分

L-glutamine: no
NaHCO3: 2.2 g/L
glucose: 3.0 g/L
phenol red: 0.011 g/L

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

McCoy 5A培养基适用于各种组织细胞原代培养。推荐用于在原代细胞培养中生产病毒。

應用

McCoy 5A培养基已用于维持:

  • G-361黑色素瘤细胞;
  • 87-MG细胞
  • 人结肠癌细胞系HCT116

生化/生理作用

McCoys 5A培养基起初是通过修改BME中的氨基酸,用来支持肝肿瘤细胞。 该配方还被用来支持骨髓、皮肤、牙龈、肾脏、大网膜、肾上腺、肺、脾、大鼠胚胎和其他细胞类型原代培养的生长。

重構

补充 0.22 g/L L-谷氨酰胺。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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