V79-4

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93010723, hamster lung, Fibroblast

• 分類程式碼代碼: 41106514

属性

• 生物源: hamster lung
• 包裝: tube of 5 μg 93010723-DNA-5UG; pkg of vial of cells 93010723-1VL
• 增長模式: Adherent
• 染色體組型: 2n = 22
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Hamster Chinese lung

細胞系描述

Derived from the lung tissue of a male Chinese hamster. The cells exhibit a high plating efficiency and a doubling time of 12-14 hours. Originally used in the discovery of X-ray induced damage and recovery in mammalian cells. This is a clone of cell line V79 (Sigma Catalogue number. 86041102).

應用

Mutagenesis and mammalian cell genetic studies

培養基

DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:6 to 1:14 i.e. seeding at 3x10,000-1x100,000 cells/cm²; 5% CO₂; 37°C using 0.25% trypsin/EDTA.

其他說明

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