DBS-FRhL-2

89101303, rhesus monkey lung, Fibroblast

• 分類程式碼代碼: 41106514

属性

• 产品名称: DBS-FRhL-2, from rhesus monkey lung, 89101303, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• 生物源: rhesus monkey lung
• 增長模式: Adherent
• 染色體組型: 2n = 42, diploid no structural abnormalities observed
• 形態學: Fibroblast
• 受體: Not specified

说明

細胞系來源

Monkey Rhesus lung

細胞系描述

A diploid cell line derived from normal male Rhesus monkey fetus. Susceptible to poliovirus types 1-3, Coxsackie A9, parainfluenza 3, rubella and is non-tumourigenic in animals.

培養基

EMEM (EBSS) (M2279) + 2 mM L-Glutamine (G7513) + 1% NEAA (M7145) + 10% FBS / FCS (F2442).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:3, i.e., seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37 °C. Finite lifespan. Cell line has been shown to undergo 51 passages or 74 population doublings.

其他說明

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法律資訊

This material is cited in a US and/or other Patent and may not be used to infringe parent claims. US Patent No. 4,040,905.