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一般說明
平衡盐溶液(BSS)用作冲洗、运输和稀释液体,同时维持细胞内和细胞外的渗透平衡。它还为细胞提供正常细胞代谢所必需的水和某些大块无机离子。BSS与碳水化合物(如葡萄糖)结合时,是细胞代谢的主要能量来源。此外,它还提供了一个缓冲系统,以维持培养基的生理pH值范围(7.2-7.6)。
應用
Hanks平衡盐已用于:
- 用于在胰腺血管分离过程中稀释消化组织
- 用于小鼠肠粘膜细胞裂解和超声前清洗
- 用作浸渍液,测试从墨鱼骨提取的多(ε-聚己内酯)-涂覆羟基磷灰石支架的体外生物活性
數量
配制成每升培养基中含粉末 9.7 克。
重構
补充 0.35 g/L 碳酸氢钠。
其他說明
加入碳酸氢钠后调节 pH 值。使用干细胞或细胞克隆的研究人员通常更喜欢不含酚红的培养基。
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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