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Merck

H1387

Sigma-Aldrich

Hanks平衡盐

Modified, without phenol red and sodium bicarbonate, powder, suitable for cell culture

别名:

HBSS

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About This Item

MDL號碼:
分類程式碼代碼:
12352207
NACRES:
NA.75

品質等級

形狀

powder

技術

cell culture | mammalian: suitable

儲存溫度

2-8°C

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一般說明

平衡盐溶液(BSS)用作冲洗、运输和稀释液体,同时维持细胞内和细胞外的渗透平衡。它还为细胞提供正常细胞代谢所必需的水和某些大块无机离子。BSS与碳水化合物(如葡萄糖)结合时,是细胞代谢的主要能量来源。此外,它还提供了一个缓冲系统,以维持培养基的生理pH值范围(7.2-7.6)。

應用

Hanks平衡盐已用于:
  • 用于在胰腺血管分离过程中稀释消化组织
  • 用于小鼠肠粘膜细胞裂解和超声前清洗
  • 用作浸渍液,测试从墨鱼骨提取的多(ε-聚己内酯)-涂覆羟基磷灰石支架的体外生物活性

數量

配制成每升培养基中含粉末 9.7 克。

重構

补充 0.35 g/L 碳酸氢钠。

其他說明

加入碳酸氢钠后调节 pH 值。使用干细胞或细胞克隆的研究人员通常更喜欢不含酚红的培养基。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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