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Merck
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M0268

Sigma-Aldrich

Eagle最低必需培养基

With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

别名:

MEM

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

品質等級

500

形狀

powder

技術

cell culture | mammalian: suitable

成分

HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
L-glutamine: yes
sodium pyruvate: no

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

Eagle最低必需培养基(MEM培养基)是使用最广泛的一种合成细胞培养基。MEM培养基已用于多种细胞的单层培养。通常用于在胎牛血清(FBS)、小牛或马血清存在下培养贴壁细胞，如成纤维细胞。

應用

Eagle最低必需培养基(MEM培养基)已用于：
  • 原代肝细胞的分离和培养
  • 培养人Ishikawa子宮內膜癌细胞(ATTC)和L-Wnt-3a细胞(ATCC)，用于分泌Wnt家族成员3a(Wnt-3a)配体
  • 培养AN3CA细胞（子宫内膜癌细胞系）和HEC1-B人细胞（子宫内膜腺癌细胞系）

特點和優勢

  • Eagle最低必需培养基(MEM培养基)含有较高浓度的氨基酸，类似于哺乳动物培养细胞的蛋白组分。
  • 去除钙成分后，培养基可用于悬浮细胞培养。

數量

按照每升培养基9.5克粉末配制。

重構

补充2.2 g/L碳酸氢钠。

其他說明

除了以粉末形式提供外，基本上与原始配方相同。

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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