CCF-STTG1

90021502, human brain, Astrocytoma

• 分類程式碼代碼: 41106514

属性

• product name: CCF-STTG1, 90021502
• 生物源: human brain
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Astrocytoma
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Caucasian astrocytoma

細胞系描述

Established from a Grade IV astrocytoma from a 68-year old Caucasian female. Cells exhibit strong perinuclear acid and phosphatase activity, glial fibrillary acidic protein present in 70-80% cells. HLA-DR present on approximately 20-30% cells after 48 hours culture.

應用

HLA profiles

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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