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一般說明
研究领域：细胞信号转导
胆固醇酯酶(CE)是一种糖蛋白，可从柱状假丝酵母和荧光假单胞菌等真菌中提取。属于脂肪酶/酯酶家族成员，以同型二聚体形式发挥作用。胆固醇酯酶在胰腺中生成，经胆囊收缩素(CCK)刺激后以活性形式释放。
胆固醇酯酶(CE)是一种糖蛋白，可从柱状假丝酵母和荧光假单胞菌等真菌中提取。属于脂肪酶/酯酶家族成员，以同型二聚体形式发挥作用。胆固醇酯酶在胰腺中生成，经胆囊收缩素(CCK)刺激后以活性形式释放。
應用
胆固醇酯酶（来自荧光假单胞菌）用于：
- 在胆固醇酯酶检测中用于定量分析人血清样本中的总胆固醇
- 脂解制备物酯酶活性的非变性蛋白电转移研究
- 酶法胆固醇测定试剂（含胆固醇氧化酶）的组分优化研究
- 修饰人血浆低密度脂蛋白(LDL)，用于在分支组织工程血管(TEBV)中诱导内皮细胞(EC)障碍和单核细胞黏附(MC)
- 在菲律宾菌素染色法检测视网膜冷冻切片CE中，水解天然胆固醇酯(CE)
该酶广泛应用于诊断实验室血清胆固醇的测定。
生化／生理作用
胆固醇酯酶(CE)是一种可水解或合成胆固醇等固醇物质脂肪酸酯部分的可逆性酶。胆固醇酯酶活性在类固醇生物合成或类固醇从头合成(de novo steroidogenesis)中起重要作用。对水不溶性长链脂肪酸酯的水解需要胆汁盐激活。对水溶性短链脂肪酸酯和溶血磷脂的水解则不需要胆汁盐激活。还可水解甘油三酯、甘油二酯和甘油一酯、磷脂、溶血磷脂和神经酰胺。该酶在脂质和脂蛋白代谢以及动脉粥样硬化中起多种作用。
其他說明
含有磷酸钾和 TRITON®X-100。
單位定義
在牛磺胆酸盐存在下，一个单位在 pH7.0，37°C 下每分钟将 1.0μ 摩尔胆固醇油酸酯水解成胆固醇和油酸。
分析報告
双缩脲法测定蛋白质。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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