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應用
当在临床分析中与胆固醇氧化酶结合使用时，该酶可用于酶法测定总胆固醇。
生化／生理作用
胆固醇酯酶（CE）是一种可逆酶，可以水解或合成胆固醇和其他固醇的脂肪酸酯。水不溶性长链脂肪酸酯的水解需要经过胆盐活化。短链脂肪酸和溶血磷脂的水溶性酯的水解则不需要胆盐的活化。它还可水解三、二和单酰基甘油、磷脂、溶血磷脂和神经酰胺。该酶在脂质和脂蛋白代谢以及动脉粥样硬化中可能具有多种功能。
物理性質
稳定性：在–20°C稳定至少一年
等电点：5.9 ± 0.1
米氏常数：5.4 x 10‾5M（亚油酸脂），6.6 x 10‾5M（油酸）
3.7 x 10‾5M（亚油酸脂），1.5 x 10‾4M（棕榈酸）
1.2 x 10‾4M（肉豆蔻），2.3 x 10‾5M（硬脂酸酯）
抑制剂：Hg++、Ag+、离子型去垢剂
最适pH值： 7.0 − 9.0
最适温度：40°C
pH稳定性： pH 5.0 − 9.0 (25°C, 24hr)
热稳定性：低于55°C（pH 7.5，10分钟）
等电点：5.9 ± 0.1
米氏常数：5.4 x 10‾5M（亚油酸脂），6.6 x 10‾5M（油酸）
3.7 x 10‾5M（亚油酸脂），1.5 x 10‾4M（棕榈酸）
1.2 x 10‾4M（肉豆蔻），2.3 x 10‾5M（硬脂酸酯）
抑制剂：Hg++、Ag+、离子型去垢剂
最适pH值： 7.0 − 9.0
最适温度：40°C
pH稳定性： pH 5.0 − 9.0 (25°C, 24hr)
热稳定性：低于55°C（pH 7.5，10分钟）
單位定義
在牛磺胆酸盐存在下，一个单位在 pH7.0，37°C 下每分钟将 1.0μmole胆固醇油酸酯水解成胆固醇和油酸。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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商品
Cholesterol undergoes esterification to improve transport. Cholesterol esters are more easily packaged into the interior of lipoproteins - increasing the quantity that can be readily transported in the blood stream.
实验方案
Protocol for Assay Procedure for Cholesterol Esterase
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