C9134

α-Chymotrypsin−Agarose from bovine pancreas

lyophilized powder, 2,000-3,500 units/g agarose (One ml gel will yield 65-120 units)

• EC 号: 3.4.21.1 (BRENDA, IUBMB)
• MDL號碼: MFCD00130481
• 分類程式碼代碼: 12352204

属性

• 生物源: bovine pancreas
• 品質等級: 200
• 形狀: lyophilized powder
• 比活性: 2,000-3,500 units/g agarose (One ml gel will yield 65-120 units)
• 分子量: 25 kDa
• UniProt登錄號: P00767
• 儲存溫度: −20°C
• 基因資訊: cow ... CTRB1(618826)

说明

應用

α-Chymotrypsin agarose from bovine pancreas has been used to study the purification and characterization of glucoamylase. α-Chymotrypsin agarose from bovine pancreas has also been used in a study to investigate molecular modeling for the design of a biomimetic chimeric ligand.

生化／生理作用

α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. The pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca²⁺ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10 mM Cu²⁺ and Hg²⁺.

Serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met) on the carboxyl end of the bond.

單位定義

One unit will hydrolyze 1.0 μmole of N-acetyl-L-tyrosine ethyl ester (ATEE) per min at pH 8.0 at 30 °C.

外觀

Stabilized with lactose

分析報告

由 A1%/280 测定的蛋白质

安全信息

• 象形圖: GHS07,GHS08
• 訊號詞: Danger
• 危險聲明: H315 - H317 - H319 - H334 - H335
• 防範說明: P280 - P302 + P352 - P305 + P351 + P338
• 危險分類: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
• 標靶器官: Respiratory system
• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 個人防護裝備: dust mask type N95 (US), Eyeshields, Faceshields, Gloves