推荐产品
生物源
mouse
品質等級
共軛
peroxidase conjugate
抗體表格
purified immunoglobulin
抗體產品種類
secondary antibodies
無性繁殖
RG-96, monoclonal
形狀
lyophilized powder
物種活性
rabbit
應無反應活性
goat, feline, pig, guinea pig, rat, bovine, canine, human, horse, sheep, chicken
包裝
vial of 0.5 mL
技術
direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200
western blot: 1:160,000 using total cell extract of HeLa cells (5-10 μg/well)
同型
IgG1
儲存溫度
2-8°C
目標翻譯後修改
unmodified
正在寻找类似产品? 访问 产品对比指南
一般說明
Anti-Rabbit IgG (γ-chain specific)-Peroxidase antibody, Mouse monoclonal is a monoclonal anti-rabbit IgG conjugated to horseradish peroxidase. Monoclonal antirabbit IgG is derived from the RG-96 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with rabbit IgG.
Rabbit IgGs are often used as primary antibodies in several immunological assays. Thus, polyclonal anti-rabbit IgGs are usually used as secondary antibodies in such applications. However, polyclonal antibodies to rabbit IgGs can also cross-react with IgGs from other species, especially with human IgGs, and thereby lead to a lack of specificity in test results. The use of monoclonal anti-rabbit IgG that is specific for only rabbit IgG improves the accuracy of immunological assays and thus, can be useful for various research applications.
The product recognizes an epitope located on the γ (heavy)-chain of rabbit IgG. In immunoblotting, the antibody recognizes both native and denatured forms of rabbit IgG. In ELISA, the antibody is specific for rabbit IgG, and shows no cross-reactivity with rabbit IgA and IgM or human IgG, IgA, and IgM. No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, or sheep.
免疫原
Rabbit IgG
應用
Monoclonal Anti-Rabbit IgG (γ-chain specific)- Peroxidase has been used in
- various immunochemical assays like enzyme linked immunosorbent assay (ELISA)
- westernblotting
- immunohistology
- dot immunobinding assay
外觀
Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.
準備報告
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
未找到合适的产品？
试试我们的产品选型工具.
訊號詞
Warning
危險聲明
危險分類
Skin Sens. 1
儲存類別代碼
13 - Non Combustible Solids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
NsrR: a key regulator circumventing Salmonella enterica serovar Typhimurium oxidative and nitrosative stress in vitro and in IFN-gamma-stimulated J774. 2 macrophages
Gilberthorpe NJ, et al.
Microbiology, 153(Pt 6), 1756-1756 (2007)
The rapid increase of circulating adiponectin in neonatal calves depends on colostrum intake
Kesser J, et al.
Journal of Dairy Science, 98(10), 7044-7051 (2015)
Targeted inhibition of EG-1 blocks breast tumor growth
Lu M, et al.
Cancer Biology & Therapy, 6(6), 936-941 (2007)
Daniel M Held et al.
Clinical and vaccine immunology : CVI, 17(6), 930-936 (2010-04-23)
New Zealand White (NZW) rabbits were immunized with several different nontoxic botulinum neurotoxin serotype B (BoNT/B) preparations in an effort to optimize the production of a rapid and highly potent, effective neutralizing antibody response. The immunogens included a recombinant heavy
A W Horne et al.
Molecular human reproduction, 15(5), 287-294 (2009-03-14)
Ectopic pregnancy (EP) remains a considerable cause of morbidity and occasional mortality. Currently, there is no reliable test to differentiate ectopic from intrauterine gestation. We have previously used array technology to demonstrate that differences in gene expression in decidualized endometrium
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门