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Merck

11170376001

Roche

抗溴脱氧尿苷

from mouse IgG1 (clone: BMC9318)

别名:

anti-BrdU, 抗体

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About This Item

分類程式碼代碼:
12352203

生物源

mouse

品質等級

共軛

unconjugated

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

BMC9318, monoclonal

化驗

90% (HPLC and SDS-PAGE)

形狀

solution

包裝

pkg of 50 μg (500 μl)

製造商/商標名

Roche

同型

IgG1

儲存溫度

−20°C

一般說明

溴脱氧尿苷抗体是5-溴-2′-脱氧尿苷-5′-单磷酸的单克隆抗体。

特異性

该抗体能够与溴脱氧尿苷特异性结合,并与碘尿苷(10%)发生交叉反应。溴脱氧尿苷抗体与氟脱氧尿苷无交叉反应,并且与任何内源性细胞组分(如胸苷或尿苷)无交叉反应。

免疫原

表位明显位于DNA螺旋内。在抗体有效结合DNA-BrdU之前,必须将DNA变性为ssDNA。

應用

溴脱氧尿苷(Anti-BrdU)抗体适用于监测血液、组织和肿瘤中的增殖细胞,以及使用以下方法检测单细胞水平的BrdU掺入:
  • 流式细胞术分析
  • 免疫组织细胞化学分析
  • 冷冻切片分析
  • 石蜡切片分析

品質

通过以考马斯蓝染色的SDS-PAGE和HPLC检测,得到抗体纯度为90%。

規格

制备:用溴脱氧尿苷-牛血清白蛋白偶联物对BALB/c小鼠进行免疫。将从脾脏中分离的淋巴细胞与Ag8.653骨髓瘤细胞融合,产生BMC 9318克隆。抗体来源于BALB/c小鼠的腹水,并通过离子交换层析进行纯化。
测试次数:250次(流式细胞术)

外觀

稳定溶于磷酸盐缓冲盐水,pH 7.4,含有0.09%(w/v)叠氮化钠和0.2%(w/v)明胶。

準備報告

工作浓度:流式细胞术:2 μg/ml (0.2 μg/100 μl/106个细胞);免疫组织细胞化学分析:6 μg/ml
偶联物的工作浓度取决于应用和底物。应使用含0.1% BSA的PBS(pH 7.4)进行稀释,以维持抗体的稳定性。

分析報告

溴脱氧尿苷抗体与碘-脱氧尿苷具有10%的交叉反应,但与氟-脱氧尿苷无交叉反应。
与任何内源性胸苷或尿苷均无交叉反应。
尚未测试其与5-Br-UTP的交叉反应,但是有研究人员认为存在交叉反应的可能性很大,因为它们的唯一区别是溴取代的远端核糖上没有羟基。

其他說明

仅用于生命科学研究。不可用于诊断。

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

No data available

閃點(°C)

No data available


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