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製造商／商標名
(BCK- EdU488FC100)
螢光
λex 496 nm; λem 516 nm
運輸包裝
wet ice
儲存溫度
2-8°C
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一般說明
Baseclick品牌的EdU流式细胞分析试剂盒(EdU Flow Cytometry Kit)为直接检测DNA合成提供了替代BrdU和[3H]胸苷检测的出色方法。EdU(5-乙炔基-2′-脱氧尿苷)是一种胸苷类似物，可在DNA合成活跃期掺入到DNA中。与溴脱氧尿苷(BrdU)检测法不同，EdU流式细胞术法不依赖抗体，因此并不需要进行DNA变性以检测掺入的核苷。恰恰相反，EdU流式细胞试剂盒利用点击化学法来检测不同种类的染料荧光示值。此外，简化的检测方案不仅减少总步骤数，而且大幅缩短总用时。这一简单的点击化学检测程序在30分钟内即可完成，兼容的多重检测可提供内容和含义丰富的结果。
應用
流式细胞术
訊號詞
Danger
危險分類
Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Muta. 1B - Repr. 2 - Skin Sens. 1
儲存類別代碼
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
其他客户在看
Yongmao Yu et al.
Journal of immunological methods, 350(1-2), 29-35 (2009-08-04)
T lymphocyte proliferations can be measured by [(3)H]thymidine incorporation. However, many labs avoid this technique because of the need to use radioactive substrates. In addition, [(3)H]thymidine incorporation method does not permit simultaneous characterization of the proliferating cells. We developed the
Fatemah Chehrehasa et al.
Journal of neuroscience methods, 177(1), 122-130 (2008-11-11)
Labelling and identifying proliferating cells is central to understanding neurogenesis and neural lineages in vivo and in vitro. We present here a novel thymidine analogue, ethynyl deoxyuridine (EdU) for labelling dividing cells, detected with a fluorescent azide which forms a
商品
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
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