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Key Documents

MABS2299

Sigma-Aldrich

Anti-Neuropilin-1 Antibody, ECD b1b2 Antibody, clone mAb3

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

mouse

品質等級

共軛

unconjugated

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

mAb3 (alternatively 7E8), monoclonal

分子量

calculated mol wt 103.13 kDa

純化經由

using protein G

物種活性

human

包裝

antibody small pack of 100 μg

技術

ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
inhibition assay: suitable

同型

IgG1κ

表位序列

Extracellular domain

Protein ID登錄號

UniProt登錄號

運輸包裝

dry ice

目標翻譯後修改

unmodified

基因資訊

human ... NRP1(8829)

一般說明

Neuropilin-1 (UniProt: O14786; also known as Vascular endothelial cell growth factor 165 receptor, CD304) is encoded by the NRP1 (also known as NRP, VEGF165R) gene (Gene ID: 8829) in human. Neuropilins are highly conserved, non-tyrosine kinase, single-transmembrane glycoproteins that serve as co-receptors for various molecules, including semaphorins and vascular endothelial growth factors (VEGF). Neuropilin-1 is a single-pass type I, homodimeric, membrane glycoprotein that is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to generate the mature form that contains a long extracellular domain (aa 22-856), a transmembrane domain (aa 857-879), and a cytoplasmic domain (aa 880-923). It acts as a receptor involved in the development of the cardiovascular system, angiogenesis, and formation of certain neuronal circuits. It is also reported to mediate the chemorepulsant activity of semaphorins. Neuropilin-1 has four well conserved domains: CUB (a1/a2; aa 27-141 and 147-265) domain, F5/8 (b1/b2; aa 275-424 and 431-583) domain, MAM domain (aa 645-811), and another domain that contains a transmembrane and a short cytoplasmic region. CUB domains mediate binding to semaphorins, while the tandem F5/8 domains are responsible for VEGF and heparin binding and recognizes a C-end role (CendR) motif R/KXXR/K on its ligands. The presence of the CUB (a1a2) domain is reported to enhance VEGF165 binding to F5/8 (b1b2) domain. The MAM domain is reported to mediate semaphorin signaling to Plexin-A. Neuropilin-1 has been recognized as an additional cellular mediator that facilitates the entry of SARS-CoV-2 into host cells. It binds to furin-cleaved spike protein and significantly potentiates SARS-CoV-2 infectivity. This effect can be blocked by monoclonal antibodies against the extracellular F5/8 (b1/b2) domain of Neuropilin-1 or with selective Neuropilin antagonists that bind to CendR binding b1 pocket. (Ref.: Kyrou, I., et al. (2021). Signal Trans. Targeted Ther. 6; Article number: 21; Daly, JL., et al. (2020). Science. 370(6518); 861-865; Cantuti-Castelvetri, L., et al. (2020). Science. 370(6518); 856-860; Mamluk, R., et al. (2002). J. Biol. Chem. 277(27); 24818-24825).

特異性

Clone mAb3 is a mouse monoclonal antibody that detects Neuropilin-1. It targets an epitope within the b1b2 domain in the extracellular region.

免疫原

His-tagged recombinant fragment corresponding to 311 amino acids from the b1b2 region within the extracellular domain of human Neuropilin-1.

應用

Quality Control Testing

Evaluated by Immunocytochemistry in PC-3 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Neuropilin-1 in PC-3 cells.

Tested Applications

Immunofluorescence Analysis: A representative lot detected Neuropilin-1 in Immunofluorescence applications (Cantuti-Castelvetri, L., et al. (2020). Science. 370(6518):856-860; Daly, J.L., et al. (2020). Science. 370(6518):861-865).

Immunocytochemistry Analysis: A representative lot detected Neuropilin-1 in Immunocytochemistry applications (Daly, J.L., et al. (2020). Science. 370(6518):861-865).

Inhibition: A representative lot detected Neuropilin-1 in Inhibition applications (Cantuti-Castelvetri, L., et al. (2020). Science. 370(6518):856-860; Daly, J.L., et al. (2020). Science. 370(6518):861-865).

ELISA Analysis: A representative lot detected Neuropilin-1 in ELISA applications (Daly, J.L., et al. (2020). Science. 370(6518):861-865).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Neuropilin-1, ECD b1b2, clone mAb3, Cat. No. MABS2299, is a mouse monoclonal antibody that detects Neuropilin-1 (NRP1) and is tested for use in ELISA, Immunocytochemistry, Immunofluorescence, and Inhibition Assay.

外觀

Purified mouse monoclonal antibody IgG1 in PBS without azide.

儲存和穩定性

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2


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