推荐产品
生物源
mouse
品質等級
抗體表格
culture supernatant
抗體產品種類
primary antibodies
無性繁殖
133A2, monoclonal
物種活性
rat, mouse, canine, bovine, human
製造商／商標名
Chemicon®
技術
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
同型
IgG3
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
unmodified
基因資訊
human ... LMNA(4000)
一般說明
Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished: A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2.
特異性
Monoclonal 133A2 reacts against human lamin A but not lamin C. The epitope lies with the 98 amino acids of the C terminus that is unique to lamin A. Specifically deletion analysis has shown that amino acids 598-611 were essential for reactivity {Hozak, P et.al. (1995), J. Cell Sci 635-644}.
免疫原
Epitope: C-terminus, a.a. 598-611
Synthetic peptide from the C-terminus of human lamin A. This sequence is not present in the the lamin C isoform.
應用
Immunoblotting: Lamin A detected as a 70kDa protein under reduced conditions.
Immunocytochemistry on fixed cells (methanol/acetone fixation)
Immunohistochemistry on frozen tissue sections.
Optimal working dilutions must be determined by the end user.
Immunocytochemistry on fixed cells (methanol/acetone fixation)
Immunohistochemistry on frozen tissue sections.
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
This Anti-Lamin A Antibody, C-terminus, a.a. 598-611, clone 133A2 is validated for use in FC, WB, IC, IH for the detection of Lamin A.
外觀
Format: Purified
Liquid in buffer containing 0.1% sodium azide.
儲存和穩定性
Maintain -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Raju Padiya et al.
PloS one, 9(5), e94228-e94228 (2014-05-07)
Cardiovascular complication due to diabetes has remained a major cause of death. There is an urgent need to intervene the cardiac complications in diabetes by nutritional or pharmacological agents. Thus the present study was designed to find out the effectiveness
Catherine Coffinier et al.
The Journal of biological chemistry, 285(27), 20818-20826 (2010-05-05)
Lamin A, a key component of the nuclear lamina, is generated from prelamin A by four post-translational processing steps: farnesylation, endoproteolytic release of the last three amino acids of the protein, methylation of the C-terminal farnesylcysteine, and finally, endoproteolytic release
J L Broers et al.
Histochemistry and cell biology, 107(6), 505-517 (1997-06-01)
A selection of normal human tissues was investigated for the presence of lamins B1, B2, and A-type lamins, using a panel of antibodies specific for the individual lamin subtypes. By use of immunoprecipitation and two-dimensional immunoblotting techniques we demonstrated that
P Hozák et al.
Journal of cell science, 108 ( Pt 2), 635-644 (1995-02-01)
The nuclear lamina forms a protein mesh that underlies the nuclear membrane. In most mammalian cells it contains the intermediate filament proteins, lamins A, B and C. As their name indicates, lamins are generally thought to be confined to the
Lamin A is part of the internal nucleoskeleton of human erythroleukemia cells.
Neri, L M, et al.
Journal of Cellular Physiology, 178, 284-295 (1999)
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