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Merck
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Key Documents

IP05

Millipore

蛋白质G Plus /蛋白质A琼脂糖悬浮液

Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.

别名:

Affinity resin, Protein G/Agarose

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About This Item

分類程式碼代碼:
41116133
NACRES:
NA.56

形狀

slurry (Liquid)

包含

≤0.1% sodium azide as preservative

製造商/商標名

Calbiochem®

儲存條件

do not freeze

技術

protein purification: suitable

適合性

suitable for microbiology

運輸包裝

wet ice

儲存溫度

2-8°C

一般說明

专为免疫沉淀应用而设计。该产品已使用 BSA 进行封闭,以减少非特异性结合,不能用于纯化。
专门配制用于免疫沉淀的蛋白 G PLUS/蛋白 A 琼脂糖混合物。

應用

免疫沉淀法(参见评论)

警告

毒性:标准处理(A)

外觀

PBS 中 33% 的浆液。

其他說明

提供琼脂糖溶液以备使用。蛋白 G Plus/蛋白 A 琼脂糖免疫沉淀试剂被 BSA 封闭,不应用于免疫球蛋白纯化或共价交联。对于免疫沉淀反应,建议每 µg 一抗加入 15 µl 溶液。预清除将最大程度地减少非特异性沉淀导致的多余条带。要进行预清除,将与免疫沉淀抗体相同物种的 20 µl 琼脂糖结合物和 1 µg 正常 IgG 加入样品中。当使用免疫印迹进行检测时,某些二抗可以与 BSA 或样品中存在的高浓度的其他蛋白质发生非特异性反应。这可以通过降低二抗的浓度来消除。

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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