推荐产品
形狀
slurry (Liquid)
包含
≤0.1% sodium azide as preservative
製造商/商標名
Calbiochem®
儲存條件
do not freeze
技術
protein purification: suitable
適合性
suitable for microbiology
運輸包裝
wet ice
儲存溫度
2-8°C
一般說明
专为免疫沉淀应用而设计。该产品已使用 BSA 进行封闭,以减少非特异性结合,不能用于纯化。
专门配制用于免疫沉淀的蛋白 G PLUS/蛋白 A 琼脂糖混合物。
應用
免疫沉淀法(参见评论)
警告
毒性:标准处理(A)
外觀
PBS 中 33% 的浆液。
其他說明
提供琼脂糖溶液以备使用。蛋白 G Plus/蛋白 A 琼脂糖免疫沉淀试剂被 BSA 封闭,不应用于免疫球蛋白纯化或共价交联。对于免疫沉淀反应,建议每 µg 一抗加入 15 µl 溶液。预清除将最大程度地减少非特异性沉淀导致的多余条带。要进行预清除,将与免疫沉淀抗体相同物种的 20 µl 琼脂糖结合物和 1 µg 正常 IgG 加入样品中。当使用免疫印迹进行检测时,某些二抗可以与 BSA 或样品中存在的高浓度的其他蛋白质发生非特异性反应。这可以通过降低二抗的浓度来消除。
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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