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化驗
>98% (HPLC and SDS-PAGE)
形狀
suspension
包裝
pkg of 15 mL (05015952001 [settled resin volume])
pkg of 2 mL (11719416001 [settled resin volume])
pkg of 5 mL (11243233001 [settled resin volume])
製造商/商標名
Roche
儲存溫度
2-8°C
一般說明
蛋白质G最初是从G148中分离出来,这是一种人组G链球菌菌株。这是一种细胞壁蛋白,有很高的IgG(免疫球蛋白G)亲和性。它公认的分子量是30000Da。它能和所有人IgG亚型互作,也能够结合小鼠、兔和山羊IgG。
特異性
蛋白质G是一种细胞壁蛋白质,从特殊细菌菌株中分离出来,对于来自不同物种的某些型免疫球蛋白有特异性结合位点。蛋白G几乎能够结合所有IgG亚型,但无法结合其它型免疫球蛋白。
结合能力:20mg人IgG(多克隆)/ml(IgG在pH7.0上样,pH2.8条件下采用20mg甘氨酸洗脱)
结构:重组蛋白G(E.coli,Mr=22000)共价偶联到4%交联琼脂糖珠:2.5mg蛋白质G(>98%纯化)/1ml胶。
结合能力:20mg人IgG(多克隆)/ml(IgG在pH7.0上样,pH2.8条件下采用20mg甘氨酸洗脱)
结构:重组蛋白G(E.coli,Mr=22000)共价偶联到4%交联琼脂糖珠:2.5mg蛋白质G(>98%纯化)/1ml胶。
應用
亲和层析使用蛋白G琼脂糖,就像蛋白A琼脂糖,是从许多物种中纯化IgG的方法。它可用作
- 泛素化实验
- 免疫沉淀实验的预清理剂
特點和優勢
内容物
Preswollen胶,即用型,2ml或5ml胶(固定胶体积),溶于含14-19%乙醇(作为保鲜剂)的缓冲溶液中,未灭菌。
蛋白G内容物:2.5mg/ml preswollen胶
Preswollen胶,即用型,2ml或5ml胶(固定胶体积),溶于含14-19%乙醇(作为保鲜剂)的缓冲溶液中,未灭菌。
蛋白G内容物:2.5mg/ml preswollen胶
品質
纯度:>98% (HPLC, SDS-PAGE); 完全没有葡萄球菌肠毒素。
其他說明
仅用于生命科学研究。不可用于诊断。
訊號詞
Warning
危險聲明
危險分類
Eye Irrit. 2 - Flam. Liq. 3
儲存類別代碼
3 - Flammable liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
102.2 °F
閃點(°C)
39 °C
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