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Key Documents

AP112A

Sigma-Aldrich

Goat Anti-Human IgG Antibody, Alkaline Phosphatase conjugate

Chemicon®, from goat

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.46

生物源

goat

品質等級

共軛

alkaline phosphatase conjugate

抗體表格

F(ab′)2 fragment of affinity isolated antibody

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

物種活性

human

製造商/商標名

Chemicon®

技術

ELISA: suitable
western blot: suitable

運輸包裝

wet ice

目標翻譯後修改

unmodified

相关类别

一般說明

Alkaline Phosphatase-conjugated Affinity Purified Goat anti-Human IgG (H+L)

特異性

Based on immunoelectrophoresis, the antibody reacts with the heavy chains on human IgG and with light chains common to most human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins, but the antibody may cross-react with immunoglobulins from other species.

應用

Detect Human IgG using this Goat anti-Human IgG Antibody, Alkaline Phosphatase conjugate validated for use in ELISA & WB.
EIA and Western blots: 1:5,000-1:50,000.

Immunohistochemistry: 1:1,000-1:2,000.

Optimal working dilutions must be determined by end user.
Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies

聯結

Replaces: MABN1055

外觀

Serum
Lyophilized. Buffer = 0.01 M Tirs HCl, 0.25 M NaCl, pH 8.0 with

15 mg/mL BSA and 0.05% sodium azide.

RECONSTITUTION:

Reconstitute with sterile distilled water to match the volume indicated on the vial label. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature.

儲存和穩定性

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. After dilution do not use for more than one day. For extended storage after reconstitution, add an equal volume of glycerol (ACS grade for better) to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

WARNING:

For research use only; not for use as a diagnostic.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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訊號詞

Warning

危險聲明

危險分類

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3


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Frontiers in immunology, 12, 765211-765211 (2021-12-28)
Saturation suppressor mutagenesis was used to generate thermostable mutants of the SARS-CoV-2 spike receptor-binding domain (RBD). A triple mutant with an increase in thermal melting temperature of ~7°C with respect to the wild-type B.1 RBD and was expressed in high
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Turkiye parazitolojii dergisi, 45(3), 201-206 (2021-08-05)
The follow-up of patients with cystic echinococcosis (CE) offers the opportunity of evaluating the prognosis of the infection as well as detecting relapse. This study aimed to evaluate the performance of the new multiepitope recombinant peptide (recDipol) antigen in the
Eleana Hatzidaki et al.
International journal of biochemistry and molecular biology, 11(1), 1-10 (2020-03-27)
Although monoclonal antibodies are promising, a truly fully human antibody is yet to be produced. Current human antibodies have the human sequence, but are produced in either transgenic animals or in phages. The aim of this paper was to produce

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