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HPLC Analysis of Methadone Enantiomers and Methadone Metabolite EDDP on Astec® CYCLOBOND I 2000 HP-RSP

HPLC Analysis of Methadone Enantiomers and Methadone Metabolite EDDP on Astec® CYCLOBOND I 2000 HP-RSP application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Astec® CYCLOBOND I 2000 HP-RSP Chiral HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

standard

Product No.
Description
Pricing

(±)-Methadone hydrochloride

powder, ≥98%

DL-Methadone-d3 solution

98 atom % D, 1 mg/mL in methanol, drug standard

CONDITIONS

column

CYCLOBOND I 2000 HP-RSP, 25 cm x 4.6 mm I.D., 5 μm particles (24024AST)

mobile phase

[A] 0.1% acetic acid; [B] acetonitrile; (80:20, A:B)

flow rate

0.8 mL/min

detector

UV, 292 nm

Description

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
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