HPLC Analysis of Benzodiazepines on Ascentis® Express Phenyl-Hexyl with Acetonitrile Mobile Phase
Materials
Analytical column
Product No.
Description
Pricing
Ascentis® Express Phenyl-Hexyl, 2.7 μm HPLC Column
2.7 μm particle size, L × I.D. 5 cm × 4.6 mmStandard
Product No.
Description
Pricing
Oxazepam solution
1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Alprazolam solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Clonazepam solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Diazepam solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express Phenyl-Hexyl, 5 cm x 4.6 mm I.D., 2.7 μm particles (53348-U)
mobile phase
[A] water; [B] acetonitrile; (62:38, A:B)
flow rate
1.4 mL/min
pressure
2600 psi (179 bar)
column temp.
35 °C
detector
UV, 250 nm
injection
2 μL
sample
0.1 g/mL of each in 20% methanol
Description
Analysis Note
The Phenyl-Hexyl phase of the Ascentis Express product line displays the best selectivity of this sample; selectivity is markedly affected by choice of organic modifier. The application also demonstrates feasibility of fast LC with Ascentis Express by virtue of low backpressure and high efficiency.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany