Poly(anhydride-ester) and poly(N-vinyl-2-pyrrolidone) blends: salicylic acid-releasing blends with hydrogel-like properties that reduce inflammation.

Poly(anhydride-ester) and poly(N-vinyl-2-pyrrolidone) blends: salicylic acid-releasing blends with hydrogel-like properties that reduce inflammation.

Macromolecular bioscience (2014-10-22)

Michelle A Ouimet, Renata Fogaça, Sabrina S Snyder, Sameer Sathaye, Luiz H Catalani, Darrin J Pochan, Kathryn E Uhrich

ABSTRACT

Polymers such as poly(N-vinyl-2-pyrrolidone) (PVP) have been used to prepare hydrogels for wound dressing applications but are not inherently bioactive. For enhanced healing, PVP was blended with salicylic acid-based poly(anhydride-esters) (SAPAE) and shown to exhibit hydrogel properties upon swelling. In vitro release studies demonstrated that the chemically incorporated drug (SA) was released from the polymer blends over 3-4 d in contrast to 3 h, and that blends of higher PVP content displayed greater swelling values and faster SA release. The polymer blends significantly the inflammatory cytokine, TNF-α, in vitro without negative effects.

MATERIALS

Product Number

Brand

Product Description

PHR1013

Supelco

Salicylic acid, Pharmaceutical Secondary Standard; Certified Reference Material

S0200000

Salicylic acid, European Pharmacopoeia (EP) Reference Standard

S-019

Supelco

Salicylic acid, 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant^®

PHR1125

Supelco

L-Glutamine, Pharmaceutical Secondary Standard; Certified Reference Material

76523

Supelco

L-Glutamine, certified reference material, TraceCERT^®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

W368401

Sigma-Aldrich

L-Glutamine

52341

Supelco

Salicylic acid, certified reference material, TraceCERT^®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

676853

Sigma-Aldrich

Dichloromethane, ACS reagent, ≥99.5%, contains 40-150 ppm amylene as stabilizer

1609002

USP

Salicylic acid, United States Pharmacopeia (USP) Reference Standard

PHR1557

Supelco

Methylene Chloride, Pharmaceutical Secondary Standard; Certified Reference Material

D65100

Sigma-Aldrich

Dichloromethane, contains 40-150 ppm amylene as stabilizer, ACS reagent, ≥99.5%

320269

Sigma-Aldrich

Dichloromethane, ACS reagent, ≥99.5%, contains 40-150 ppm amylene as stabilizer

32222

Sigma-Aldrich

Dichloromethane, puriss. p.a., ACS reagent, reag. ISO, ≥99.9% (GC)

494453

Sigma-Aldrich

Dichloromethane, biotech. grade, 99.9%, contains 40-150 ppm amylene as stabilizer

24233

Sigma-Aldrich

Dichloromethane, puriss., meets analytical specification of Ph. Eur., NF, ≥99% (GC)

650463

Sigma-Aldrich

Dichloromethane, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains 50-150 ppm amylene as stabilizer

G8540

Sigma-Aldrich

L-Glutamine, meets USP testing specifications, suitable for cell culture, 99.0-101.0%, from non-animal source

G6392

Sigma-Aldrich

L-Glutamine, γ-irradiated, BioXtra, suitable for cell culture

W296600

Sigma-Aldrich

Pyridine, ≥99%

270970

Sigma-Aldrich

Pyridine, anhydrous, 99.8%

165212

Sigma-Aldrich

Adipoyl chloride, 98%

439223

Sigma-Aldrich

Dichloromethane, suitable for HPLC, ≥99.9%, contains 40-150 ppm amylene as stabilizer

276855

Sigma-Aldrich

Dimethyl sulfoxide, anhydrous, ≥99.9%

270997

Sigma-Aldrich

Dichloromethane, anhydrous, ≥99.8%, contains 40-150 ppm amylene as stabilizer

07-4872

Sigma-Aldrich

Dimethyl sulfoxide, ≥99.0%, suitable for absorption spectrum analysis

07-4875

Sigma-Aldrich

Dimethyl sulfoxide, ≥99.5%

07-2180

Sigma-Aldrich

Dichloromethane, JIS special grade, ≥99.0%

07-4860

Sigma-Aldrich

Dimethyl sulfoxide, JIS special grade, ≥99.0%

07-4870

Sigma-Aldrich

Dimethyl sulfoxide, SAJ first grade, ≥99.0%

24-6500

Sigma-Aldrich

Pyridine, JIS special grade, ≥99.5%