ZRB1912

Anti-PAK-1 Antibody, clone 1H5 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Serine/threonine-protein kinase PAK 1;EC:2.7.11.1;Alpha-PAK;p21-activated kinase 1;PAK-1;p65-PAK
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1H5, recombinant monoclonal
• description: 1H5 Clone
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 60.65 kDa; observed mol wt ~60 kDa
• purified by: using Protein A
• species reactivity: rat, human
• species reactivity (predicted by homology): bovine, mouse, canine, porcine, feline, monkey
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): affinity binding assay: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal half
• Protein ID accession no.: NP_002567
• UniProt accession no.: Q13153
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... PAK1(5058)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1H5 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects PAK-1. It targets an epitope within 15 amino acids from the N-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal half of human PAK-1.

Application

Quality Control Testing

Evaluated by Western Blotting in C6 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PAK-1 in C6 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PAK-1 in Human brain and Rat brain tissue lysates.

Affinity Binding Assay: A representative lot of this antibody bound PAK-1 peptide with a KD of 1.3 x 10-6 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected PAK-1 in human stomach tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in C6 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PAK-1 in C6 cell lysate.

Target description

Serine/threonine-protein kinase PAK 1 (UniProt: EC:2.7.11.1, Alpha-PAK, p21-activated kinase 1, PAK-1, p65-PAK) is encoded by the PAK1 gene (Gene ID: 5058) in human. PAK-1 is a serine/threonine protein kinase that is involved in intracellular signaling pathways downstream of integrins and receptor-type kinases. It is in active conformation in its monomeric form and is autoinhibited upon homodimerization. It plays an important role in cytoskeleton dynamics, cell adhesion, migration, proliferation, and apoptosis. It is also involved in vesicle-mediated transport processes. It is involved in the reorganization of the actin cytoskeleton, actin stress fibers and of focal adhesion complexes. Its protein kinase domain is localized in amino acids 270-521. PAK-1 is activated by interaction with Cdc42 and Rac1. It functions as a GTP effector that links Cdc42 and Rac1 to JNK MAP kinase pathway. It phosphorylates and activates MAP2K1, and thereby mediates activation of downstream MAP kinases. It is reported to directly phosphorylate BAD and protect cells against apoptosis. In cells undergoing apoptosis, its activity is inhibited due to binding of CDK11A and CDK11B. PAK-1 is reported to phosphorylate RAF1 at serine 338 and 339 that results in RAF1 activation and translocation to mitochondria where it can phosphorylate BAD. PAK-1 is activated following autophosphorylation at threonine 423 in response to a conformation change that is triggered by interaction with GTP-bound Cdc42 or Rac1. Binding of GTP-boundCdc42 or Rac1 to its autoregulatory region (aa 70-140) releases monomers from the autoinhibited homodimeric state and enables phosphorylation (at Thr 423) and activation. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Harms, FL., et al. (2018). Am. J. Hum. Genet. 103(4); 579-591; Zenke, FT., et al. (1999). J. Biol. Chem. 274(46); 32565-32573; Sells, MA., et al. (1997). Curr. Biol. 7(3); 202-210).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1