ZRB1251

Anti-Chromogranin A Antibody, clone 5F23 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 5F23, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 50.69 kDa; observed mol wt ~54 kDa
• purified by: using Protein A
• species reactivity: human
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal half
• Protein ID accession no.: NP_001266.1
• UniProt accession no.: P10645
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 5F23 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Chromogranin A. It targets an epitope within the C-terminal half.

Immunogen

His-tagged recombinant fragment corresponding to 183 amino acids from the C-terminal half of human Chromogranin A.

Application

Quality Control Testing

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Chromogranin A in A549 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Chromogranin A in HepG2 cell lysate.

Affinity Binding Assay: A representative lot of this antibody bound Chromogranin A with a KD of 3.3 x 10-7 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Chromogranin A in human pancreas tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Chromogranin A in SH-SY5Y cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Chromogranin A, clone 5F23 ZooMAb^®, Cat. No. ZRB1251, is a recombinant Rabbit monoclonal antibody that specifically targets Chromogranin A and is tested in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Target description

Chromogranin-A (UniProt: P10645; also known as CgA, Pituitary secretory protein I, SP-I) is encoded by the CHGA gene (Gene ID: 1113) in human. Chromogranin-A is a secreted protein that is synthesized with a signal peptide aa 1-18), which is subsequently cleaved off to produce the mature form that is further cleaved into 18 different chains with various biological activities. Notably amongst these are Pancreastatin (aa 272-319) that inhibits glucose induced insulin release from the pancreas, Catestain (aa 370-390) that blocks catecholamine release from chromaffin cells and noradrenergic neurons, and Serpinin (aa 429-454) that regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1. It may be secreted in the blood as a full-length protein or in fragments after cleavage. Due to its primary expression throughout the neuroendocrine system, it has been widely accepted biomarker for the assessment of neuro-endocrine tumors. It has also been correlated with a several non-malignant systemic disorders, such as hypertension, cardiac, and hepatic failure. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.(Ref.: Gkolfinopoulos, S., et al. (2017). World J. Methodol. 7(1); 9-15).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb^® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable