ZMS1089
Anti-Ubiquitin Lys63 Antibody, clone HWA4C4 ZooMAb® Mouse Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Ub
About This Item
Recommended Products
biological source
mouse
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
HWA4C4, recombinant monoclonal
product line
ZooMAb® learn more
form
lyophilized
purified by
using protein G
species reactivity
human
packaging
antibody small pack of 25 μL
greener alternative product characteristics
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enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
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technique(s)
ELISA: suitable
affinity binding assay: suitable
western blot: suitable
isotype
IgG2aλ
epitope sequence
N-terminal half
Protein ID accession no.
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... UBB(7314)
General description
Specificity
Immunogen
Application
Evaluated by Western Blotting in lysate from HeLa cells treated with MG132.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Ubiquitin Lys63 in lysate from HeLa cells treated with MG132 (25 nM; 4 h), but not in untreated cells.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Ubiquitin Lys63 in Ubiquitin K63, Ubiquitin K11, and Ubiquitin K48.
Affinity Binding Assay: A representative lot of this antibody bound recombinant Ubiquitin-Lys63 with a KD of 5.2 x 10-6 in an affinity binding assay.
Enzyme Immunoassay Analysis (ELISA): A serial of dilutions from a representative lot detected recombinant Ubiquitin Lys63, but not Ubiquitin K11 or GST protein.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in lysate from HeLa cells treated with MG132.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Ubiquitin Lys63 in lysate from HeLa cells treated with MG132 (25 nM; 4 h), but not in untreated cells.
Target description
Physical form
Reconstitution
Storage and Stability
Legal Information
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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