Y2021

Yeast Synthetic Drop-out Medium Supplements

without histidine, leucine, tryptophan, and adenine

• Synonym(s): Yeast Synthetic Drop-out Media Supplements
• MDL number: MFCD01095679
• UNSPSC Code: 12352200
• NACRES: NA.85

Properties

• Quality Level: 200
• form: powder
• technique(s): transformation: suitable
• application(s): microbiology
• storage temp.: room temp

Description

Application

Yeast Synthetic Drop-out Medium Supplements, without histidine, leucine, tryptophan, and adenine has been used in yeast two-hybrid screening.

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Other Notes

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Preparation Note

Dissolve 1.39 g of supplement per liter of medium.

Safety Information

• pictograms: GHS05
• signalword: Danger
• hcodes: H318
• pcodes: P280 - P305 + P351 + P338
• Hazard Classifications: Eye Dam. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable