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V9764

Sigma-Aldrich

Anti-VPS34 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Vps34 Antibody, Vps34 Antibody - Anti-VPS34 antibody produced in rabbit, Anti-PIK3C3, Anti-Phosphoinositide-3-kinase, Anti-Vacuolar protein sorting 34

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~105 kDa

species reactivity

mouse, rat, human

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: suitable
western blot: 2-4 μg/mL using whole extracts of HEK-293T cells expressing human VPS34

UniProt accession no.

Q8NEB9

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PIK3C3(5289)
mouse ... Pik3c3(225326)
rat ... Pik3c3(65052)

General description

Vacuolar protein sorting 34 (VPS34), also known as phosphatidylinositol 3-kinase catalytic subunit type 3 (PIK3C3), is encoded by the gene mapped to human chromosome 18q12.3. The encoded protein belongs to the phosphoinositide (PI)-3-kinase family. VPS34 is widely expressed in all tissues of mammals.

Immunogen

synthetic peptide corresponding to amino acids 22-34 of human VPS34, conjugated to KLH via an added cysteine residue. The corresponding sequence is identical in mouse and rat.

Application

Anti-VPS34 antibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

Vacuolar protein sorting 34 (VPS34) is a lipid kinase that phosphorylate phosphatidylinositol (PtdIns), to form phosphatidylinositol 3 phosphate (PtdIns3P). VPS34 plays a vital role in various intracellular trafficking events such as autophagy and phagosome formation. Thus, the inhibition of kinase activity of Vps34 might be considered as a potential therapeutic method for cancer treatment. VPS34 is also involved in internal vesicle formation within multivesicular endosomes, reverse transport of endosome to Golgi apparatus and membrane transport from the outer nuclear membrane towards the cell periphery. Mutation in the gene encoding VPS34 is associated with pathogenesis of schizophrenia. VPS34 plays an essential role in nutrient/amino acid induced activation of mechanistic target of rapamycin (mTOR) signaling in cultured cells and it also facilitates early embryogenesis and cell proliferation.

Target description

VPS34 is known to be involved in endocytosis and vesiculartrafficking.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The phosphoinositide (PI) 3-kinase family.
Fiona M Foster et al.
Journal of cell science, 116(Pt 15), 3037-3040 (2003-06-28)
Ruqi Tang et al.
Neuroscience letters, 437(1), 42-44 (2008-04-19)
The PIK3C3 gene has been implicated as a candidate gene for schizophrenia by functional evidence and genetic association studies. A series of previous studies have found susceptibility SNPs in promoter region. To further verify its susceptibility to schizophrenia in the
Xiang Zhou et al.
PloS one, 6(1), e16358-e16358 (2011-02-02)
The Pik3c3 gene encodes an 887 amino acid lipid kinase, phosphoinositide-3-kinase class 3 (PIK3C3). PIK3C3 is known to regulate various intracellular membrane trafficking events. However, little is known about its functions during early embryogenesis in mammals. To investigate the function
Benoit Pasquier et al.
Journal of medicinal chemistry, 58(1), 376-400 (2014-11-18)
Vps34 (the human class III phosphoinositide 3-kinase) is a lipid kinase involved in vesicle trafficking and autophagy and therefore constitutes an interesting target for cancer treatment. Because of the lack of specific Vps34 kinase inhibitors, we aimed to identify such
Elma Aflaki et al.
Aging cell, 15(1), 77-88 (2015-10-22)
Gaucher disease, the inherited deficiency of lysosomal glucocerebrosidase, is characterized by the presence of glucosylcer-amide macrophages, the accumulation of glucosylceramide in lysosomes and the secretion of inflammatory cytokines. However, the connection between this lysosomal storage and inflammation is not clear.
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