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T0910

Sigma-Aldrich

Thioredoxin from Escherichia coli

recombinant, expressed in E. coli, essentially salt-free, lyophilized powder, ≥3 units/mg protein

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About This Item

CAS Number:
52500-60-4
MDL number:
MFCD00132423
UNSPSC Code:
12352200
NACRES:
NA.32

biological source

Escherichia coli

Quality Level

200

recombinant

expressed in E. coli

assay

≥90% (SDS-PAGE)

form

essentially salt-free, lyophilized powder

specific activity

≥3 units/mg protein

mol wt

11.7 kDa

technique(s)

activity assay: suitable

solubility

water: 1.90-2.10 mg/mL, clear, colorless

Protein ID accession no.

M26133

UniProt accession no.

P0AA25

application(s)

cell analysis

storage temp.

−20°C

Gene Information

Escherichia coli K12 ... trxA(948289)

General description

Research area: Cell Signaling

Thioredoxin from Escherichia coli is a monomeric, globular protein. It has a central β-sheet surrounded by α-helices.

Application

Thioredoxin from Escherichia coli has been used in thioredoxin reductase assay, peroxidase-coupled thioredoxin system assay and peroxiredoxin assay. It has also been used to study its effects on photoreceptor death in a mouse model of retinitis pigmentosa (RP) and its potential to modify retinal oxidative stress, glial changes, and vascular changes during the progression of the disease.

Biochem/physiol Actions

Thioredoxin from Escherichia coli catalyzes REDOX reactions and has a role in various biological processes. It modulates the structure and activity of many proteins. The enzyme thioredoxin reductase catalyzes the reduction of thioredoxin. Thioredoxin also has a role in oxidative stress response.Thioredoxin, known as both adult T cell leukemia-derived factor (ADF) and a cellular redox enzyme, functions as a cytokine or chemokine, facilitating cell-to-cell communication. Additionally, it plays a role in regulating cell growth and apoptosis.
Mediates the reduction of disulfide bonds in proteins.

Other Notes

GenBank® Accession Number M26133.

Unit Definition

One unit will cause a ΔA650 of 1.0 in 1 min at 25 °C in the insulin reduction assay.

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Thioredoxin from Escherichia coli as a Role Model of Molecular Recognition, Folding, Dynamics and Function.
Vazquez DS
Protein and peptide letters, 22(9), 801-815 (2015)
Proteomic analysis of thioredoxin-targeted proteins in Escherichia coli.
Kumar JK
Proceedings of the National Academy of Sciences of the USA, 101(11), 3759-3764 (2004)
Mechanism and structure of thioredoxin reductase from Escherichia coli.
Williams CH Jr
Faseb Journal, 9(13), 1267-1276 (1995)
Ohr (organic hydroperoxide resistance protein) possesses a previously undescribed activity, lipoyl-dependent peroxidase.
Cussiol JR
The Journal of Biological Chemistry, 285(29), 21943-21950 (2010)
Jérôme Delroisse et al.
Antioxidants (Basel, Switzerland), 10(8) (2021-08-28)
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