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Key Documents

SRP4363

Sigma-Aldrich

VEGF165 human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

Synonym(s):

Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen, VPF, Vascular Endothelial Growth Factor

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About This Item

MDL number:
MFCD00286542
UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

1.0-7.0 ng/mL

mol wt

~38.2 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

impurities

endotoxin, tested

NCBI accession no.

Q96KJ0

UniProt accession no.

P15692

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... VEGFA(7422)

General description

The gene VEGFA (vascular endothelial growth factor A) is a member of VEGF family of growth factors. The gene is localized to human chromosome 6p21.1. It contains eight exons interspaced by seven introns, spanning a length of 14 kb. The critical region of 2.36 kbp in human promoter lacks a consensus TATA box. However, it contains various consensus-binding sites for many transcriptional regulators.

Biochem/physiol Actions

The gene VEGFA (vascular endothelial growth factor A) encodes a secreted mitogen that is found to participate in physiological and pathological angiogenesis. It serves as a growth factor for endothelial cells and functions in the formation of new blood vessels during embryonic development and adulthood. It binds to two tyrosine kinase receptors VEGFR1 and VEGFR2 and functions in angiogenesis and maintenance of vasculature.

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
Reconstitute in water to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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VEGFA is necessary for chondrocyte survival during bone development.
Zelzer E
Development, 131, 2161-2171 (2004)
Processing of VEGF-A by matrix metalloproteinases regulates bioavailability and vascular patterning in tumors.
Lee S
The Journal of Cell Biology, 169, 681-691 (2005)
Genetic variants on chromosome 6p21.1 and 6p22.3 are associated with type 2 diabetes risk: a case-control study in Han Chinese.
Lu F
Journal of Human Genetics, 57, 320-325 (2012)
Vivian Lehmann et al.
Biotechnology progress, 35(2), e2745-e2745 (2018-11-14)
The only cure available for Type 1 diabetes involves the transplantation of islets of Langerhans isolated from donor organs. However, success rates are relatively low. Disconnection from vasculature upon isolation and insufficient rate of revascularization upon transplantation are thought to

Articles

VEGF Pathway

Read review on VEGF signaling pathway (Vascular endothelial growth factor (VEGF)/VEGF receptor) and find related products.

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