SRP3062
IGF-Binding Protein 1 human
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym(s):
IBP-1, Placenta Protein 12 (PP12)
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About This Item
UNSPSC Code:
51111800
NACRES:
NA.32
Recommended Products
biological source
human
recombinant
expressed in E. coli
assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
potency
<0.5 μg/mL
mol wt
25.4 kDa
packaging
pkg of 25 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... IGFBP1(3484)
General description
Insulin-like growth factor-binding proteins (IGF-BPs) form high affinity complexes with both insulin-like growth factor- I and II (IGF-I and IGF-II). Currently there are seven named IGF-BPs. Insulin-like growth factor-binding protein-1 (IGF-BP1) is a 25.4kDa cysteine-rich secreted protein. It is the most abundant IGF-BP in amniotic fluid and is expressed in liver, decidua and kidneys. Levels of IGF-BP1 in serum are lowest after food. It binds to both IGF-I and IGF-II with equal affinity. Phosphorylated IGF-BP1 hinders IGF actions, whereas non-phosphorylated IGF-BP1 is stimulatory. Recombinant human IGF-BP1 is a 25.4kDa protein consisting of 235 amino acid residues (Isoform A).
Biochem/physiol Actions
Insulin-like growth factor-binding proteins (IGF-BPs) control the distribution, function and activity of insulin-like growth factors (IGFs) in various cell tissues and body fluids. They prolong the half-life of IGFs. Insulin-like growth factor-binding protein-1 (IGF-BP1) has been shown to have a role in the development and advancement of cancers. It is downregulated in hepatocellular carcinoma.
Sequence
MAPWQCAPCS AEKLALCPPV SASCSEVTRS AGCGCCPMCA LPLGAACGVA TARCARGLSC RALPGEQQPL HALTRGQGAC VQESDASAPH AAEAGSPESP ESTEITEEEL LDNFHLMAPS EEDHSILWDA ISTYDGSKAL HVTNIKKWKE PCRIELYRVV ESLAKAQETS GEEISKFYLP NCNKNGFYHS RQCETSMDGE AGLCWCVYPW NGKRIPGSPE IRGDPNCQIY FNVQN
Physical form
Lyophilized from 10 mM Sodium Phosphate, pH 7.5.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Hiroyasu Kamei et al.
PloS one, 3(8), e3091-e3091 (2008-09-05)
Gene duplication is the primary force of new gene evolution. Deciphering whether a pair of duplicated genes has evolved divergent functions is often challenging. The zebrafish is uniquely positioned to provide insight into the process of functional gene evolution due
Over-expression of insulin-like growth factor binding protein-related protein-1(IGFBP-rP1/mac25) in the M12 prostate cancer cell line alters tumor growth by a delay in G1 and cyclin A associated apoptosis.
Ezzat V.A., et al.
Diabetes, obesity & metabolism, 3, 198-211 (2008)
Carnosine decreases IGFBP1 production in db/db mice through suppression of HIF-1.
Forsberg EA
The Journal of Endocrinology, 225(3), 159-167 (2015)
The extracellular regulation of growth factor action.
Flaumenhaft R
Molecular Biology of the Cell, 3(10), 1057-1065 (1992)
C Mannelli et al.
Mediators of inflammation, 2014, 635364-635364 (2014-04-17)
A proper fetomaternal immune-endocrine cross-talk in pregnancy is fundamental for reproductive success. This might be unbalanced by exposure to environmental chemicals, such as bisphenol A (BPA). As fetoplacental contamination with BPA originates from the maternal compartment, this study investigated the
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