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Key Documents

SAB4701040

Sigma-Aldrich

Monoclonal Anti-BrdU antibody produced in mouse

clone Bu20a

Synonym(s):

5-bromodeoxyuridine

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

Bu20a, monoclonal

form

buffered aqueous solution

concentration

1 mg/mL

technique(s)

flow cytometry: 2 μg/mL

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

The antibody Bu20a reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It is useful for detecting proliferating cells by flow cytometry or immunohistochemistry staining.

Immunogen

Bromodeoxyuridine conjugated to BSA

Physical form

Solution in phosphate buffered saline with 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jan-Bernd Stukenborg et al.
Journal of andrology, 29(3), 312-329 (2007-11-30)
Isolation and culture of spermatogonial stem cells (SSCs) has become an approach to study the milieu and the factors controlling their expansion and differentiation. Traditional conventional cell culture does not mimic the complex situation in the seminiferous epithelium providing a

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