SAB4200764

Anti-Dystrophin antibody, Mouse monoclonal

clone MANDYS8, purified from hybridoma cell culture

• Synonym(s): Anti-DMD
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: MANDYS8, monoclonal
• form: buffered aqueous solution
• mol wt: ~427 kDa
• species reactivity: porcine, rabbit, mouse, rat, human
• enhanced validation: independent; Learn more about Antibody Enhanced Validation
• concentration: ~1.0 mg/mL
• technique(s): immunoblotting: suitable; immunohistochemistry: 5-10 μg/mL using acetone fixed rat tongue frozen sections
• isotype: IgG2b
• UniProt accession no.: P11532
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... DMD(1756)

Description

General description

Anti-Dystrophin antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the MANDYS8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mouse immunized with fragment of recombinant human dystrophin. Dystrophin is a 427 kD protein which joins the actin cytoskeleton to laminin. It possesses multiple domains and is localized to the extracellular matrix. Dystrophin contains NH2-terminal domain for actin binding. The C-terminal domain interacts with dystrophin associated protein (DAP).

Dystrophin is a rod-shaped cytoskeletal protein located to the periphery (plasma membrane) of normal striated muscle fibers.

Specificity

Anti-Dystrophin antibody, Mouse monoclonal specifically recognizes an epitope located on the rod domain of the human dystrophin molecule.

Immunogen

recombinant human dystrophin

Application

Anti-Dystrophin antibody, Mouse monoclonal has been used in immunoblotting and immunohistochemistry.

Biochem/physiol Actions

Dystrophin is known to prevent muscle fibre injury due to contraction.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable