SAB4200609

Monoclonal Anti-diMethyl-Histone H3 (diMe-Lys⁹) (H3K9me2) antibody produced in mouse

~1.0 mg/mL, clone 5E5-G5, purified immunoglobulin

• Synonym(s): Anti-H3F3A H3.3A H3F3 PP781 H3F3B H3.3B, Anti-HIST1H3A H3FA, Anti-HIST1H3B H3FL, Anti-HIST1H3C H3FC, Anti-HIST1H3D H3FB, Anti-HIST1H3E H3FD, Anti-HIST1H3F H3FI, Anti-HIST1H3G H3FH, Anti-HIST1H3H H3FK, Anti-HIST1H3I H3FF, Anti-HIST1H3J H3FJ, Anti-HIST2H3A HIST2H3C H3F2 H3FM HIST2H3D
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 5E5-G5, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~17 kDa
• concentration: ~1.0 mg/mL
• technique(s): immunoblotting: 2-4 μg/mL using histones isolated from human HeLa cells.; immunofluorescence: 1-2 μg/mL using human HeLa cells.
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: dimethylation (Lys9)

Description

General description

Monoclonal Anti-diMethyl-Histone H3 (diMe-Lys9) (H3K9me2) (mouse IgG1 isotype) is derived from the hybridoma 5E5-G5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a dimethylated (diMe-Lys9) peptide corresponding to the N-terminus of human histone H3, conjugated to KLH. Histones H3 and H4 are the core histones forming nucleosome, which is the fundamental unit of chromatin. H3 and H4 remodified by methylation and are highly methylated in mammalian cells.

Immunogen

dimethylated (diMe-Lys⁹) peptide corresponding to the N-terminus of human histone H3, conjugated to KLH. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Application

Monoclonal Anti-diMethyl-Histone H3 (diMe-Lys9) (H3K9me2) antibody produced in mouse may be used in several immunochemical techniques including immunoblotting (~17 kDa) and immunofluorescence.

Biochem/physiol Actions

Histones are subjected to extensive covalent modifications, including phosphorylation, methylation, acetylation and ubiquitination thought to play an important role in development and in cancer. Histone methylation, is a complex, dynamic process involving various biological processes including transcriptional regulation, chromatin condensation, mitosis and heterochromatin assembly. Moreover, lysine residues can be mono-, di-, and tri-methylated, adding further complexity to the regulation of chromatin structure. Conserved lysine residues in the N-terminal tail domains of histone H3, Lys⁴, Lys⁹ and Lys²⁷ are the preferred sites of methylation. Methylation of H3 at Lys⁹ is a modification intrinsically linked to epigenetic silencing and heterochromatin assembly.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable