SAB4200399

Anti-WIPI-2 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-ATG18B, Anti-Atg21, Anti-CGI-50, Anti-WIPI2, WD repeat domain, phosphoinositide interacting 2
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~49 kDa
• species reactivity: rat, mouse, human
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 2.5-5.0 μg using lysates of rat NRK cells.; western blot: 1-2 μg/mL using using whole extracts of HEK-293T cells over-expressing mouse WIPI-2.
• UniProt accession no.: Q9Y4P8
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... WIPI2(26100) ; mouse ... Wipi2(74781) ; rat ... Wipi2(288498)

Description

General description

The WIPI2 gene is mapped on the human chromosome at 7p22.1. WIPI2 has a 7-bladed propeller structure and contains a conserved motif.

WD repeat domain, phosphoinositide-interacting 2 (WIPI-2) belongs to the WIPI protein family. It is localized on the autophagy membrane and plasma membrane. Apart from this, it is also present on the membranes near the Golgi cisternae. WIPI2 is a mammalian ortholog of Atg18, which is ubiquitously expressed in a variety of cell lines.

Specificity

Anti-WIPI-2 recognizes human, rat, and mouse WIPI-2.

Application

Anti-WIPI-2 antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

WIPI-2 (WD repeat domain, phosphoinositide-interacting 2) plays a vital role in the transformation of omegasomes into autophagosomes. The above protein functions as a mammalian effector of phosphatidylinositol 3-phosphate (PtdIns3P). WIPI2 aids recombinant capsid protein VP1 (rVP1) in upregulating autophagy, MAPK1 (Mitogen-activated protein kinase 1)/3 phosphorylation and matrix metallopeptidase 9 (MMP-9) activity, which are required for macrophage migration.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable