P302-05

Porcine Pulmonary Artery Endothelial Cells: PPAEC (Cryovial)

• UNSPSC Code: 41106514
• NACRES: NA.81

Properties

• biological source: Porcine pulmonary artery (Normal healthy)
• Quality Level: 100
• form: solid
• packaging: pkg of 500,000 cells
• manufacturer/tradename: Cell Applications, Inc
• growth mode: Adherent
• karyotype: 2n = 38
• morphology: Endothelial
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): diabetes; cardiovascular diseases
• shipped in: dry ice
• storage temp.: −196°C

Description

General description

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PPAEC have been used in a pioneer study of endothelial cell motility, where it was determined that asymmetric dimethylarginine (ADMA), an inhibitor of nitric oxide production and a cardiovascular risk factor, decreased PKG-mediated phosphorylation of VASP, leading to downregulation of Rac1 and induction of stress fibers and focal adhesions via RhoA/ROCK dependent mechanism, leading to decreased cell motility, and increase in endothelial permeability, thus linking vascular repair and function with NO production (Wojciak-Stothard, 2007, 2009). PPAEC were also used to assess endothelial adhesion to decellularized porcine arteries in order to develop grafts for human vascular repair (Xiong, 2013).

Cell Line Origin

Artery

Application

cardiovascular function, screening of drug candidates, angiogenesis, vasodilator function, transport of deoxygenated bloood from heart to lungs

Components

Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO

Preparation Note

• 1st passage, >500,000 cells in Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO
• Can be cultured at least 16 doublings

Subculture Routine

Please refer to the PPAEC Culture Protocol.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable