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N1905

Sigma-Aldrich

Neurotrophin-3 human

recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture, >97% (SDS-PAGE)

Synonym(s):

HDNF, NTF3, NT-3

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About This Item

MDL number:
MFCD00212404
UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

Quality Level

200

recombinant

expressed in baculovirus infected Sf21 cells

assay

>97% (SDS-PAGE)

form

lyophilized powder

potency

1-40 ng/mL EC50

packaging

pkg of 5 μg

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

P20783

storage temp.

−20°C

Gene Information

human ... NTF3(4908)

General description

NTF3 (neurotrophin 3) is a neurotrophic factor belonging to the NGF (nerve growth factor) family. This protein is made up of 257 amino acids, with a prepro region of 138 amino acids and the mature sequence of 119 residues.

Application

Neurotrophin-3 human has been used in the magnetic sorting of oligodendrocyte progenitor cells.

Biochem/physiol Actions

Neurotrophin-3 (13.6 kDa or 27.2 kDa dimer) is a member of the neurotrophin family. It has identical amino acid sequence in human, mouse, and pig with full cross-reactivities. NT-3 is important in development and maintenance of neuronal populations and promotes differentiation of neural crest derived sensory and sympathetic neurons. It is critical for proprioceptive 1a afferent neurons, which relay information from peripheral muscle spindles to motoneurons, sending projections to spinocerebellar neurons. NT-3 is also critical in the superior cervical and nodose ganglia.

Physical form

Lyophilized from a 0.2 m filtered solution in phosphate buffered saline (PBS) containing 50 μg of bovine serum albumin (BSA) per 1 μg of cytokine.

Analysis Note

The biological activity is measured in a cell proliferation assay using a TrkB-transfected cell line BaF-TrkB-BD.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Neurotrophin-3 modulates breast cancer cells and the microenvironment to promote the growth of breast cancer brain metastasis
Louie E, et al.
Oncogene, 32(35), 4064-4064 (2013)
Defective glial maturation in vanishing white matter disease
Bugiani M, et al.
Journal of Neuropathology and Experimental Neurology, 70(1), 69-82 (2011)
Linkage of the gene for the triple A syndrome to chromosome 12q13 near the type II keratin gene cluster
Weber A, et al.
Human Molecular Genetics, 5(12), 2061-2066 (1996)
Neurotrophin 3 is a mitogen for cultured neural crest cells.
Kalcheim C et al
Proceedings of the National Academy of Sciences of the USA, 89(5), 1661-1665 (1992)
Cloning and expression of a cDNA encoding a novel human neurotrophic factor.
Kaisho Y et al
Febs Letters, 266(1-2), 187-191 (1990)
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