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MBD6009

Sigma-Aldrich

5R-PLEX beads

for PCR purification

Synonym(s):

5R-PLEX SPRI beads

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.55

Quality Level

200

form

suspension

technique(s)

PCR: suitable

storage temp.

2-8°C

General description

Solid Phase Reversible Immobilization (SPRI) beads are magnetic beads that can be utilized for DNA purification and size selection. The 5R-PLEX beads are SPRI beads designed to easily separate the desired PCR amplicon from nucleotides, adapters, primers, and primer dimers by efficient purification and size selection. The magnetic beads are provided in a ready to use suspension that enables fast and efficient application even in high throughput applications with automated liquid handling. The beads are formulated with NaCl and high purity PEG as the crowding agent.

Application

  • NGS library preparation, including 16S and 18S
  • Approved to use in ultrasensitive 5R-Plex (SMURF) library preparation(2,3,4) for degraded samples
  • PCR reaction clean up
  • Amplicon size selection
  • DNA purification and manipulation (replacing elution buffer, concentrating the sample)

Features and Benefits

  • Ready to use
  • Compatible with automated liquid handling
  • Easy size selection by changing the volumetric ratio of beads to samples

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

Certificates of Analysis (COA)

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Lishay Parhi et al.
Nature communications, 11(1), 3259-3259 (2020-06-28)
Fusobacterium nucleatum is an oral anaerobe recently found to be prevalent in human colorectal cancer (CRC) where it is associated with poor treatment outcome. In mice, hematogenous F. nucleatum can colonize CRC tissue using its lectin Fap2, which attaches to

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