MAK475

Creatinine Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• UNSPSC Code: 12352204

Properties

• Quality Level: 100
• detection method: colorimetric; fluorometric
• relevant disease(s): renal disease; neurological disorders; genitourinary disorders
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Creatinine is synthesized in the body from creatine, which is produced from creatine phosphate during muscle contractions. In the blood, creatinine is removed by filtration through the glomeruli of the kidney and is secreted into urine. In healthy individuals, creatinine secretion is independent of diet and is fairly constant. The creatinine clearance test has become one of the most sensitive tests for measuring glomerular filtration rate. In kidney disease, creatinine levels in the blood are elevated, whereas the creatinine clearance rate and subsequent creatin levels in urine are diminished. Creatinine testing is most widely used to assess kidney function.

Features and Benefits

• The linear detection range of creatinine is 4.8 to 500 µM (0.054 - 5.7 mg/dL) for the colorimetric assay and 0.25 to 100 µM (0.0028 - 1.14 mg/dL) for the fluorometric assay.
• Compatible with high-throughput handling systems.
• Convenient. The procedure involves adding a single working reagent and reading after 60 minutes.

Suitability

The kit is suitable for creatinine determination in urine, serum, plasma, and other biological preparations.

Principle

The Creatinine Assay Kit uses a reaction sequence to convert a dye into a colored and fluorescent form. The absorbance at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is directly proportional to the creatinine concentration in the sample. The reaction specifically excludes both endogenous creatine and ammonia.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3