MAK122
Phospholipid Assay Kit
sufficient for 100 colorimetric or fluorometric tests
Synonym(s):
Phospholipid Quantification Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
usage
sufficient for 100 colorimetric or fluorometric tests
application(s)
cosmetics
food and beverages
pharmaceutical
detection method
colorimetric
fluorometric
shipped in
dry ice
storage temp.
−20°C
Related Categories
General description
Phospholipids are a class of lipids which constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline.
In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2-specific dye. This results in a colorimetric (570 nm)/ fluorometric (λex = 530/λem = 585 nm) product directly proportional to the phospholipid concentration in the sample. The range of linear detection is 3 - 200 μM for colorimetric assays and 0.6 - 20 μM for fluorometric assays.
In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2-specific dye. This results in a colorimetric (570 nm)/ fluorometric (λex = 530/λem = 585 nm) product directly proportional to the phospholipid concentration in the sample. The range of linear detection is 3 - 200 μM for colorimetric assays and 0.6 - 20 μM for fluorometric assays.
Application
Phospholipid assay kit has been used in biochemical analysis and phospholipid quantitation.
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for the detection of phospholipids in biological samples
Principle
In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2-specific dye. This results in a colorimetric (570 nm)/ fluorometric (λex = 530/λem = 585 nm) product directly proportional to the phospholipid concentration in the sample. The range of linear detection is 3 - 200 μM for colorimetric assays and 0.6 - 20 μM for fluorometric assays.
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - Resp. Sens. 1
Storage Class
10 - Combustible liquids
Certificates of Analysis (COA)
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