M1196

Anti-Mucolipin-2 (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-MCOLN2, Anti-MLN2, Anti-TRPML2
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~60 kDa
• species reactivity: human
• packaging: antibody small pack of 25 μL
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~1 mg/mL
• technique(s): western blot: 0.3-0.6 μg/mL using HEK-293T cells expressing human mucolipin-2
• UniProt accession no.: Q8IZK6
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MCOLN2(255231)

Description

General description

Mucolipin-2 (MLN2) is a member of transient receptor potential (TRP) protein superfamily. It consists of gated, tetrameric cation channels. MLN2 has a conserved structure of six transmembrane helices with differing cytoplasmic oriented N- and C-terminal domains. It is localized to late endosomes and lysosomes..

Specificity

Additional bands may be observed at ~120 kDa and ~35 kDa due to aggregation and degradation, respectively.

Immunogen

synthetic peptide corresponding to amino acids 534-549 of human mucolipin-2. This sequence has low homology with rat (73% identity) and mouse (66% homology) mucolipin-2.

Application

Anti-Mucolipin-2 (C-terminal) antibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

Mucolipin-2 (MLN2) plays an important role in lysosome maturation, lysosome fission, late endosome-lysosome fusion, autophagosome-lysosome fusion, lysosome expulsion and iron release. It stimulates autophagy proliferation via transcription factor EB (TFEB) activation. MLN2 is involved in B‐lymphocyte development.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)