Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

L1668

Sigma-Aldrich

Lectin from Phaseolus vulgaris (red kidney bean)

Phytohemagglutinin PHA-P, lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl), BioReagent, suitable for cell culture

Synonym(s):

Phaseolus vulgaris agglutinin, PHA

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

Quality Level

200

product line

BioReagent

form

lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl)

potency

≤10 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)

technique(s)

cell culture | mammalian: suitable

color

white to off-white

solubility

PBS: 5 mg/mL, clear to slightly hazy, colorless to faintly yellow (pH 6.8)

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

Application

Lectin from Phaseolus vulgaris (red kidney bean) has been used:
  • for the stimulation of cluster of differentiation (CD4+) T cells
  • for the activation of primary peripheral blood lymphocytes
  • for the stimulation of peripheral blood mononuclear cells

Biochem/physiol Actions

PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Packaging

Sold on the basis of lectin content

Analysis Note

Potency and solubility expressed in mg lectin/ml

Related product

Product No.
Description
Pricing

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Primate immunodeficiency virus proteins Vpx and Vpr counteract transcriptional repression of proviruses by the HUSH complex
Yurkovetskiy L, et al.
Nature microbiology, 1-1 (2018)
J L D'Amato et al.
Poultry science, 89(5), 938-947 (2010-04-08)
Arginine is an essential amino acid in Aves and is also an important substrate for the immune system. Dietary Arg in avian diets must be sufficient to not only support growth but also immunity. To better understand Arg needs for
Posttranscriptional Regulation of HIV-1 Gene Expression during Replication and Reactivation from Latency by Nuclear Matrix Protein MATR3
Sarracino A, et al.
mBio, 9(6), e02158-e02118 (2018)
Wesley de Jong et al.
Vaccines, 7(4) (2019-12-11)
Therapeutic vaccinations aim to re-educate human immunodeficiency virus (HIV)-1-specific immune responses to achieve durable control of HIV-1 replication in virally suppressed infected individuals after antiretroviral therapy (ART) is interrupted. In a double blinded, placebo-controlled phase IIa multicenter study, we investigated
Rapid Flow Cytometry-Based Test for the Diagnosis of Lipopolysaccharide Responsive Beige-Like Anchor (LRBA) Deficiency
Gamez-Diaz L, et al.
Frontiers in Immunology (2018)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service