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I8904

Sigma-Aldrich

Insulin-like Growth Factor-II from mouse

>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder

Synonym(s):

IGF-II

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About This Item

MDL number:
MFCD00241895
UNSPSC Code:
12352202
NACRES:
NA.77

product name

Insulin-like Growth Factor-II from mouse, IGF-II, recombinant, expressed in E. coli, lyophilized powder

biological source

mouse

recombinant

expressed in E. coli

assay

>97% (SDS-PAGE)

form

lyophilized powder

quality

endotoxin tested

mol wt

~7.4 kDa

packaging

pkg of 5X50 μg
pkg of 50 μg

impurities

<1.0EUper1mg (LAL test)

UniProt accession no.

P09535

storage temp.

−20°C

Gene Information

mouse ... IGF2(16002) , Igf2(16002)

General description

Recombinant Mouse IGF-II is produced from a DNA sequence encoding the mature IGF-II protein. Mouse IGF-II, a 67 amino acid protein, has a predicted molecular matrix of ~7.4 kDa. Mouse and human IGF-II share 91% sequence homology. Insulin-like growth factor II (also known as multiplication stimulating activity or MSA) and insulin-like growth factor I (IGF-I) belong to the family of insulin-like growth factors, which are structurally homologous to proinsulin. Mature IGF-I and IGF-II are highly conserved and share ~70% amino acid sequence identity. They have autocrine, paracrine, and endocrine functions.

Biochem/physiol Actions

Insulin-like growth factor II (IGF-II) is a potent mitogenic growth factor that mediates growth-promoting activities in embryonic development. IGF-II binds the IGF-II receptor with high affinity. IGF-I and IGF II are expressed in many tissues and cell types. IGF-II is mitogenic for a variety of cultured cells including human or chicken fibroblasts, mouse 3T3 cells, normal rat kidney.

Physical form

Lyophilized from a 0.2 μm filtered solution of 30% acetonitrile and 0.1% of TFA.

Analysis Note

The biological activity is measured in a serum-free cell proliferation assay using the human breast carcinoma cell line MCF-7.

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Biyun Qian et al.
Oncology reports, 25(1), 203-213 (2010-11-27)
High expression of insulin-like growth factor-II (IGF-II) in epithelial ovarian cancer is associated with aggressive disease and poor prognosis. IGF-II transcription is initiated from multiple promoters. Promoter-specific expression is regulated by DNA methylation, which is often dysregulated in cancer. Here
S D O'Dell et al.
The international journal of biochemistry & cell biology, 30(7), 767-771 (1998-09-02)
Insulin-like growth factor II (IGF-II) plays a key role in mammalian growth, influencing foetal cell division and differentiation and possibly metabolic regulation. The mature 67 amino acid peptide shares sequence homology with both insulin and IGF-I. The liver is the
Shuo Chen et al.
Cell reports, 32(6), 108007-108007 (2020-08-14)
Stem cell niches provide a microenvironment to support the self-renewal and multi-lineage differentiation of stem cells. Cell-cell interactions within the niche are essential for maintaining tissue homeostasis. However, the niche cells supporting mesenchymal stem cells (MSCs) are largely unknown. Using

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