H9034
Hepatitis C virus core antigen
~1 mg/mL, ≥95% (SDS-PAGE), recombinant, expressed in E. coli
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recombinant
expressed in E. coli
Quality Level
assay
≥95% (SDS-PAGE)
mol wt
22 kDa (plus 114 kDa galactosidse)
concentration
~1 mg/mL
shipped in
dry ice
storage temp.
−20°C
Gene Information
hepatitis C virus ... HCVgp1(951475)
human ... HBVgp4(944568)
General description
Hepatitis C virus core antigen (HCV-cAg) is a well-preserved antigenic protein of the internal capsid.
Hepatitis C virus glycoprotein 1 (HCVgp1), also referred to as polyprotein, is crucial for the induction of double-membrane vesicles, sites of HCV RNA amplification. The amino terminal of polyprotein contains the structural proteins core, envelope protein 1 (E1) and E2. The proteolytic cleavage of polyprotein results in the formation of 10 mature viral proteins which are needed for viral replication and assembly.
amino acids 2-192 of HCV polyprotein
Specificity
Reacts strongly with human HCV positive serum.
Application
Hepatitis core antigen (HCA) has been used as an analyte in enzyme-linked immunosorbent assay (ELISA) to study the mechanism of bovine serum albumin (BSA) blocking in common ELISA protocols.
Biochem/physiol Actions
Hepatitis C virus core antigen (HCV-cAg) may be a less expensive way to diagnose active hepatitis C virus (HCV) infection in the hemodialysis (HD) population.
Positive control for HCV antibodies.
Other Notes
HCV core antigen [2-192]-Galactosidase-tagged at the N-terminus.
Physical form
Solution in 8 M Urea, 20 mM Tris-HCl, pH 8.0, 10 mM 2-mercaptoethanol.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Margaret A Scull et al.
PLoS pathogens, 11(11), e1005297-e1005297 (2015-11-21)
The hepatitis C virus (HCV) p7 protein is required for infectious virus production via its role in assembly and ion channel activity. Although NMR structures of p7 have been reported, the location of secondary structural elements and orientation of the
Inés Romero-Brey et al.
mBio, 6(4), e00759-e00759 (2015-07-15)
Induction of membrane rearrangements in the cytoplasm of infected cells is a hallmark of positive-strand RNA viruses. These altered membranes serve as scaffolds for the assembly of viral replication factories (RFs). We have recently shown that hepatitis C virus (HCV)
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