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Key Documents

G1288

Sigma-Aldrich

β-(1→3,4,6)-Galactosidase, positionally specific

recombinant, expressed in E. coli, buffered aqueous solution

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About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.32

recombinant

expressed in E. coli

Quality Level

form

buffered aqueous solution

specific activity

≥70 units/mg protein

packaging

vial of 0.24 unit

shipped in

wet ice

storage temp.

2-8°C

Biochem/physiol Actions

Releases β(1→3)-, β(1→4)-, and β(1→6)-linked galactose from the non-reducing end of complex oligosaccharides.

Unit Definition

One unit will hydrolyze 1 μmole of p-nitrophenyl β-D-galactopyranoside per min at pH 5.0 at 37 °C.

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, 25 mM NaCl

Preparation Note

Produced from a mixture of enzymes from Streptococcus pneumonia and Xanthamonas sp.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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William C Hines et al.
Nature communications, 6, 6927-6927 (2015-04-22)
Clinically relevant human culture models are essential for developing effective therapies and exploring the biology and etiology of human cancers. Current breast tumour models, such as those from oncogenically transformed primary breast cells, produce predominantly basal-like properties, whereas the more

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