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Key Documents

G1146

Sigma-Aldrich

ʟ-Glutamine–Penicillin–Streptomycin

solution, suitable for cell culture, BioReagent

Synonym(s):

Pen/Strep, Penicillin/Streptomycin 

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About This Item

MDL number:
MFCD00283636
UNSPSC Code:
12352207
NACRES:
NA.76

product name

L-Glutamine–Penicillin–Streptomycin solution, with 200 mM L-glutamine, 10,000 U penicillin and 10 mg steptomycin/mL in 0.9% NaCl, 0.1 μm filtered, BioReagent, suitable for cell culture

biological source

synthetic

Quality Level

300

sterility

0.1 μm filtered

product line

BioReagent

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria

mode of action

cell wall synthesis | interferes
protein synthesis | interferes

shipped in

dry ice

storage temp.

−20°C

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Application

Used to reduce or eliminate bacterial contamination in cell, especially primary cell, cultures. Recommended for use in cell culture applications at 10 ml/L.

Biochem/physiol Actions

Mode of Action: Glutamine is essential as an energy source for most mammalian cells in culture because of its amino acid carbon skeleton. Penicillin acts by inhibiting bacterial cell wall synthesis. Streptomycin inhibits prokaryote protein synthesis by preventing the transition from initiation complex to chain-elongating ribosome and causes miscoding.
Antimicrobial spectrum: Gram-negative and Gram-positive bacteria.

Components

Formulated with 200 mM L-glutamine, 10,000 units/ml penicillin, and 10 mg/ml streptomycin in 0.9% sodium chloride.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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