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Key Documents

FG1383

SAFC

RPMI-1640 Medium

HEPES modification, with stable glutamine, liquid, sterile-filtered, suitable for cell culture

Pharma Manufacturing

Synonym(s):

Roswell Park Memorial Institute 1640 medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

product name

RPMI-1640 Medium, HEPES Modification, With 25 mM HEPES and stable glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered
sterile

form

liquid

technique(s)

cell culture | mammalian: suitable

components

stable glutamine: yes
HEPES: 25 mM
phenol red: yes
NaHCO3: yes

shipped in

ambient

storage temp.

2-8°C

Application

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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