F6796
Anti-Fibroblast Growth Factor Receptor-2, Extracellular antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-FGFR-2
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About This Item
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biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 115-125 kDa (doublet)
species reactivity
human
packaging
antibody small pack of 25 μL
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using trypsin-digested, human and animal tissue sections
western blot: 1:2,000 using extract of FGFR-2 transiently transfected cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... FGFR2(2263)
General description
Fibroblast growth factors (FGFs) belongs to the large tyrosine kinase family of growth factor receptors (17-38kDa). The FGF family consists of at least seventeen members designated FGF-1 through FGF-17 that bind to high affinity FGF receptors (FGFRs). Soluble, secreted or possibly cleaved forms of FGFR-1 and FGFR-2 have also been found in body fluids. They are glycosylated 110-150 kDa proteins that are constructed of an extracellular ligand binding region with either two, or typically three, immunoglobulin (Ig)-like domains and an eight amino acid "acidic box", a transmembrane region and a cytoplasmic split tyrosine kinase domain that is activated following ligand binding and receptor dimerization. The ligand binding site of all FGFRs is confined to the extracellular Ig-like domains 2 and 3.
Specificity
The antibody detects human FGFR-2 (bek) in extracts of transfected cells.
The antibody specifically reacts with human FGFR-2; no reaction with FGFR-1 or FGFR-3 is detected.
Immunogen
synthetic peptide corresponding to amino acids 362-374 of the extracellular region of human FGFR-2 (Bek).
Application
Anti-Fibroblast Growth Factor Receptor-2, Extracellular antibody produced in rabbit has been used in immunoprecipitation and immunoblotting.
Biochem/physiol Actions
Fibroblast growth factors (FGFs) are potent physiological regulators normal development, wound healing and repair, angiogenesis, a variety of neurotrophic activities, in hematopoiesis as well as in tissue remodeling and maintenance. FGFRs exhibit overlapping recognition and redundant specificity. Signal transduction by FGFRs requires dimerization or oligomerization and autophosphorylation of the receptors through their tyrosine kinase domain. Subsequent association with cytoplasmic signaling molecules leads to DNA synthesis or differentiation. The signaling and biological responses elicited by distinct FGFRs substantially differ. FGFR-2 is highly expressed in developing human tissues including the brain (preferentially in glial cells), choroid plexus, skin, lung, kidney and bone. It may be found in several anchorage-dependent cells, such as normal and malignant breast cancer cells.
Physical form
Solution in 0.01 M phosphate buffered saline,
pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
Preparation Note
Affinity isolated on a peptide-agarose column.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificates of Analysis (COA)
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D Givol et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 6(15), 3362-3369 (1992-12-01)
Since 1989, the receptors for fibroblast growth factors (FGFs) were cloned and characterized as a subgroup of the family of receptor tyrosine kinases. Four FGF receptor genes were identified, all of which encode membrane-bound glycoproteins containing three immunoglobulin (Ig) -like
Ternary complex formation of EphA4, FGFR and FRS2$\alpha$ plays an important role in the proliferation of embryonic neural stem/progenitor cells
Sawada T, et al.
Genes Cells, 15(3), 297-311 (2010)
Fibroblast growth factor 23 reduces expression of type IIa Na+/Pi co-transporter by signaling through a receptor functionally distinct from the known FGFRs in opossum kidney cells
Yan X, et al.
Genes Cells, 10(5), 489-502 (2005)
A Baird
Current opinion in neurobiology, 4(1), 78-86 (1994-02-01)
Although first characterized by virtue of their ability to stimulate endothelial cell proliferation in vitro and angiogenesis in vivo, the fibroblast growth factors are now also well recognized for their neurotrophic activities. Understanding the physiological significance of these multifunctional, virtually
Takahiro Sawada et al.
Genes to cells : devoted to molecular & cellular mechanisms, 15(3), 297-311 (2010-02-27)
EphA4 belongs to a superfamily of receptor tyrosine kinases and interacts with several molecules including fibroblast growth factor receptors (FGFRs) as we reported earlier. Several receptor tyrosine kinases, FGFRs, Trks, Alk and Ret, are currently known to transduce a signal
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