EC100
GenElute™-E Single Spin Blood DNA Kit
Synonym(s):
GenElute™-E Blood DNA Kit
About This Item
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purified by
(Single-spin negative chromotography)
(Time: 45 minutes or less)
feature
Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS)
Intended use (For the purification of genomic DNA from whole or dried blood samples)
Typical/expected yield (Varies by sample. Please reference user guide for more information.)
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storage temp.
2-8°C
General description
- Isolation of DNA in under an hour with minimal sample handling
- Better purity, leading to improved performance in PCR and other applications
- Significant reduction in plastic and hazardous chemical waste
Traditional silica-based, bind-wash-elute purification kits require multiple wash steps to remove impurities from the spin columns. These steps increase the risk of cross contamination, subject the DNA to centrifugation sheering forces, and introduce chaotropic salts that can carry over into the final sample, inhibiting downstream applications.
GenElute™-E kits employ size exclusion negative chromatography to separate large nucleic acid molecules from smaller protein, lipid, and ionic components in cell, tissue, blood, and other samples. Single-spin columns efficiently absorb and retain cellular debris and sample contaminants while allowing nucleic acids to pass through, reducing the number of steps and plastic materials required for purification. This novel method for high-quality purification is made possible by our innovative SmartLyse® Protease, which enables fast and efficient lysis of a wide range of sample types.
Isolate nucleic acid in a fraction of time compared to traditional silica bind-wash-elute procedures. Simply mix sample with lysis components and incubate according to kit protocol, pipette sample onto the spin column, and centrifuge directly into a collection tube. Cellular debris and contaminants remain bound in the column to be discarded, while purified DNA is ready to be used in downstream applications or stored.
Application
The initial blood lysis step takes place under physiological conditions where the enzyme activity of proteases in general is increased. SmartLyse® Protease contains a unique combination of several active enzymes with an expanded substrate specificity. This optimization allows for fast and efficient lysis of blood cells (from, e. g., human, animal, fish, and birds) and dried blood spots (coated FTA cards) with the same protocol.
Purified genomic DNA is eluted in Tris buffer, pH 7.8 and can immediately be used for most common downstream applications, including PCR, genotyping, NGS, and others. Final yield is comparable to most common silica-based methods. GenElute™-E purified genomic DNA preparations commonly show an A260/280 ratio of around 1.8.
Features and Benefits
Other Notes
Legal Information
signalword
Danger
Hazard Classifications
Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
Certificates of Analysis (COA)
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